Cold atmospheric plasma for surface disinfection : a promising weapon against deleterious meticillin-resistant Staphylococcus aureus biofilms

Lunder, Manca (avtor), Dahle, Sebastian (avtor), Fink, Rok (avtor)

Background: Bacteria are becoming increasingly resistant to classical antimicrobial agents, so new approaches need to be explored. Aim: To assess the potential of cold atmospheric plasma for the management of methicillin-resistant Staphylococcus aureus (MRSA). Methods: The 24, 48, and 72 h resistant and susceptible S. aureus biofilms were exposed to 60, 120, and 180 s treatment with plasma. Findings: Increasing the treatment time results in higher cell reduction for both susceptible and resistant strains of S. aureus (P < 0.05). Up to log$_{10}$ reduction factor of 5.24 cfu/cm$^2$ can be achieved in 180 s of plasma treatment. Furthermore, plasma can substantially alter the cell’s metabolisms and impact cell membrane integrity. However, it has not been shown that plasma can reduce biofilm biomass in the case of 24 h and 48 h biofilms, although the 72 h biofilm was more susceptible, and its biomass was decreased (P < 0.05). The accumulation of intrabacterial reactive oxygen species was also observed, which confirms the plasma’s induction of oxidative stress. Finally, it was shown that continuous plasma exposure of bacterial cells does not cause resistance to plasma, nor is resistance developed to cefoxitin. Conclusion: Cold atmospheric plasma is a good candidate for S. aureus and MRSA biofilm treatment and may therefore be of value in the bacterial resistance crisis.

• Jezik: Angleški jezik
• Ključne besede: cold atmospheric plasma, Staphylococcus aureus, MRSA, reduction, metabolic activity, resistance
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta ; BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 64-75
• Številčenje: Vol. 143
• PID: 20.500.12556/RUL-152544
• UDK: 616.9:614
• ISSN pri članku: 0195-6701
• DOI: 10.1016/j.jhin.2023.10.014
• COBISS.SI-ID: 173756675
• Datum objave v RUL: 27.11.2023

Gradivo je del revije

• Naslov: The journal of hospital infection
• Skrajšan naslov: J. hosp. infect.
• Založnik: Elsevier, The Healthcare Infection Society
• ISSN: 0195-6701
• COBISS.SI-ID: 6404871

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: hladna atmosferska plazma, Staphylococcus aureus, MRSA, zmanjšanje, presnovna aktivnost, odpornost

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0015
• Naslov: Les in lignocelulozni kompoziti