Nutrition is an undeniable part of promoting health and performance among football players. This is the first study to investigate the effect of a nutritional intervention on the nutritional status of professional football players in Slovenia. The study involved 14 football players from the same football club. Before the start of the nutritional intervention, they all completed a 3-day food record and physical activity diary. Fat free mass (FFM) and fat body mass were measured by bioelectrical impedance. We evaluated their dietary intake using Prodi and calculated their energy availability (EA) through energy expenditure, energy intake and FFM. We compared the data obtained with the UEFA dietary recommendations for football players during pre-season and found that their dietary intake was suboptimal. Football players were randomised into an intervention group (N = 7) and a control group (N = 7). The nutritional intervention lasted six weeks and consisted of five nutrition education sessions attended by the intervention group. After the intervention, the football players again completed a 3-day food record and physical activity diary. The Shapiro-Wilk test, Mann-Whitney U test and Wilcoxon signed-rank test were used for statistical analysis. Statistical analysis showed no statistically significant differences between the EA of the intervention and control groups after the intervention (p = 0.225). The EA of the intervention group increased after the intervention, but the difference was not statistically significantly above the recommended value (p = 0.612). The intake of carbohydrates and fat did not differ statistically significantly between the groups (p = 0.073; p = 0.249). The intake of carbohydrates and protein decreased statistically significantly in the control group after the intervention (p = 0.046; p = 0.034). The intake of fat also decreased but did not reach a statistically significant difference to the intake before the intervention (p = 0.063).
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