Ugotavljanje učinkovitosti razkuževanja kopalne vode v bazenih s postopkom elektrolize : magistrsko delo

Zorec, Stanka (avtor), Oder, Martina (mentor), Poljšak, Borut (recenzent)

Uvod: Postopek za pripravo kopalne vode imenujemo dezinfekcija oziroma razkuževanje. Eden izmed njih je kemični postopek razkuževanja kopalne vode z elektrolizo natrijevega klorida (NaCl). Učinkovitost razkužila izrazimo kot Ct, kjer je "C" koncentracija razkužila (mg/L) in "t" kontaktni čas za inaktivacijo določenega odstotka mikroorganizma. Namesto odstotka se uporablja vrednost log10, ki se imenuje logaritemska inaktivacija zapisana kot N0/Nt = e^(k*t). Namen: Namen magistrskega dela je preveriti ali postopek elektrolize NaCl povzroči nastajanje klora v kopalni vodi in tako omogoči ustrezno vzdrževanje higienskega stanja kopalne vode v daljšem obdobju. Prav tako želimo dokazati učinkovitost razkuževanja z upadom testnih bakterij E. coli in E. faecalis za tri logaritemske stopnje v bazenu zasebne uporabe z volumnom 32 m3. Metode dela: Uporabili smo testne bakterijske kulture E. coli in E. fecalis, s katerimi smo kontaminirali kopalno vodo v bazenu. Suspenziji testnih bakterij E. coli in E. faecalis za kontaminacijo bazena so pripravili v laboratoriju Veterinarske enote Slovenske vojske (SV). Odvzeli smo 6 vzorcev (V0 do V6). V0 je predstavljal vzorec vode pred izvedbo poskusa, V1 pred kontaminacijo bazena, V2 eno uro po kontaminaciji ter V3, V4 in V5 po vklopu elektrolize. Določevanje števila bakterij je potekalo v laboratoriju Veterinarske enote SV. Rezultati: Vzorec V0 je skladen s Pravilnikom in predstavlja vzdrževanje higienskega stanja kopalne vode. Postopek dezinfekcije je učinkovit, saj se je število bakterij E. coli in E. faecalis zmanjšalo za tri logaritemske stopnje v 44 minutah pri 0,15 mg/L prostega klora. Razprava in zaključek: Elektroliza z NaCl zagotovi nastajanje prostega klora. Potrdili smo, da je koncentracija prostega klora v kopalni vodi skladna z določili Pravilnika in omogoča razkuževanje z rezidualnim učinkom ob korekciji vrednosti pH in doslednim in natančnim upoštevanjem navodil proizvajalcev. S postopkom razkuževanja s postopkom elektrolize NaCl je število bakterij E. coli in E. faecalis upadlo za tri logaritemske stopnje in je kopalna voda skladna s Pravilnikom o minimalnih higienskih zahtevah, ki jih morajo izpolnjevati kopališča in kopalna voda v bazenih (Uradni list RS, št. 59/15, 86/15-popr. in 52/18).

• Jezik: Slovenski jezik
• Ključne besede: magistrska dela, sanitarno inženirstvo, elektroliza s soljo, razkuževanje z elektrolizo natrijevega klorida, E. coli, E. faecalis, logaritemska inaktivacija, metoda razkuževanja, prosti klor
• Vrsta gradiva: Magistrsko delo/naloga
• Tipologija: 2.09 - Magistrsko delo
• Organizacija: ZF - Zdravstvena fakulteta
• Kraj izida: Ljubljana
• Založnik: [S. Zorec]
• Leto izida: 2023
• Št. strani: 56 str., [9] str. pril.
• PID: 20.500.12556/RUL-151165
• UDK: 614
• COBISS.SI-ID: 167081987
• Datum objave v RUL: 30.09.2023

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Determinantig the effectiveness of disinfection in swimming pool with electrolysis : master thesis

Izvleček

Introduction: The process of preparing pool water is called disinfection. One of them is the chemical disinfection of bathing water with the electrolysis of sodium chloride (NaCl). The efficiency of the disinfectant is expressed as Ct, where "C" is the concentration of the disinfectant (mg/L), and "t" is the contact time for the inactivation of a certain percentage of microorganisms. Instead of a percentage, the log10 value is used, which is called logarithmic inactivation, written as N0/Nt = e^(k*t). Objective: The purpose of this master's thesis is to investigate whether the NaCl electrolysis process causes the formation of chlorine in bathing water and thus enables the proper maintenance of the hygienic condition of the bathing water over an extended period. We also aim to demonstrate the effectiveness of disinfection by a reduction of test bacteria E. coli and E. faecalis by three logarithmic stages in a privately owned pool with a volume of 32 m3.Methods: We used test bacterial cultures E. coli and E. faecalis to contaminate the pool water. The suspension of test bacteria E. coli and E. faecalis for pool contamination was prepared in the laboratory of the Veterinary Unit of the Slovenian Armed Forces (SV). We took 6 samples (V0 to V6). V0 represented the water sample before the experiment, V1 before pool contamination, V2 one hour after contamination, and V3, V4, and V5 after turning on the electrolysis. Bacterial counts were determined in the laboratory of the Veterinary Unit SV. Results: Sample V0 complies with the regulations and represents the maintenance of the hygienic condition of the pool water. The disinfection process is effective since the number of bacteria E. coli and E. faecalis decreased by three logarithmic stages in 44 minutes at 0.15 mg/L of free chlorine. Discussion and Conclusion: Electrolysis with NaCl ensures the formation of free chlorine. We have confirmed that the concentration of free chlorine in the pool water complies with the regulations and allows disinfection with a residual effect, with pH value correction and strict adherence to manufacturer's instructions. Using the electrolysis NaCl disinfection process, the number of bacteria E. coli and E. faecalis decreased by three logarithmic stages, and the bathing water complies with the regulations on minimal hygiene requirements for swimming pools and bathing water (Official Gazette of the Republic of Slovenia, No. 59/15, 86/15-corr., and 52/18).

• Ključne besede: master's theses, sanitary engineering, salt electrolysis, disinfection with electrolysis of sodium chloride, E. coli, E. faecalis, logarithmic inactivation, disinfection method, free chlorine