The toxicity of fiber has been shown to be related to the potential biodurability of respired fiber in the lung. The toxicological effect is best predicted in the traditional and long -term in vivo assays, but there is an increasing interest in replacement, reduction, and refinement of alternatives to animal testing. The potential solubility of fiber can be assessed by in vitro acellular test mimicking the lung environment. The use of simulated lung fluids is a promising in vitro method to better understand the release mechanisms and predicted in vivo fiber behaviour. In recent years, scientists have tested various simulated lung fluids, so many different versions of the fluids can be found in the literature. The aim of this work is to determine which fluid best mimics real biological conditions and provides robustness and reproducibility to move away from animal testing as soon as possible.
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