Prisotnost erikoidne mikorize pri komercialno dostopnih rastlinah družine vresovk (Ericaceae)

Godec, Vanessa (author), Vodnik, Dominik (mentor)

Za rastline iz družine Ericaceae je značilno, da tvorijo simbiotično povezavo z erikoidnimi mikoriznimi (ErM) glivami, ki jim omogoča oskrbo z mineralnimi hranili ter nudi zaščito pred stresom. Ta simbioza je za vresovke nujna, saj rastejo v kislih tleh z veliko nerazgrajene organske snovi, z majhno razpoložljivostjo esencialnih makroelelemntov in hkrati z veliko razpoložljivostjo potencialno strupenih kovin. ErM glive kolonizirajo lasaste korenine gostitelja in v tla sproščajo zunajcelične encime, ki razgrajujejo organsko snov ter sproščajo mineralna hranila (npr. dušik), ki jih gliva posreduje gostitelju v zameno za ogljikove hidrate. Micelij ErM ščiti rastlino pred toksičnimi kovinami. Cilje naloge je bil proučiti prisotnost ErM pri okrasnih vresovkah. Ker je znano, da se mikoriza razvije slabše, če tla vsebujejo velike količine razpoložljivih mineralnih hranil, smo predvidevali, da ErM bo prisotna, a v manjšem obsegu, saj so te rastline ob vzgoji gnojene. Vzorčili smo korenine 11 različnih vrst/hibridov vresovk iz Arboretuma Volčji potok in eno naravno rastočo vrsto (navadna borovnica, Vaccinium myrtillus). Vzorce korenin smo očistili, pobarvali z barvilom tripan modro, stopnjo infekcije pa ocenili pod mikroskopom. V sklopu naloge smo uspeli pregledati korenine 8 vrst/hibridov in ugotovili, da je mikoriza pri njih prisotna v velikem obsegu. Stopnja kolonizacije je bila različna, največja je bila pri V. myrtillus in Rhododendron impeditum, pri katerih smo v koreninah našli tudi precej temnih septiranih endofitov. Andromeda polyfolia je imela najmanj mikorize, infekcije s temnimi septiranimi endofiti pri njej nismo zaznali.

• Language: Slovenian
• Keywords: mikoriza, okrasne rastline, Ericaceae, mikorizna infekcija
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: BF - Biotechnical Faculty
• Year: 2023
• PID: 20.500.12556/RUL-150412
• COBISS.SI-ID: 164966403
• Publication date in RUL: 17.09.2023

Secondary language

• Language: English
• Title: Presence of ericoid mycorrhizae in commercially available plants of the heath family (Ericaceae)

Abstract

Plants in the Ericaceae family are characterised by forming a symbiotic relationship with ericoid mycorrhizal (ErM) fungi to obtain mineral nutrients and to protect themselves from stress. The symbiotic relationship is essential for these plants because they grow in acidic soils with a lot of undecomposed organic matter, low level of available macroelements, and high level of available toxic metals. ErM fungi colonise hairy roots and release extracellular enzymes that break down soil organic material and mobilise nutrients (e.g., nitrogen) that are passed on to their symbiotic partners in exchange for carbohydrates. The objective of this study was to analyse the presence of ericoid mycorrhizae in ornamental ericaceous plants. Since mycorrhiza is known to occur to a lesser extent in soils that are well supplied with readily available nutrients, we assumed that it will be present but to a much lesser extent because these plants are fertilised. We sampled roots of 11 different ericaceous plant species/hybrids in the Arboretum Volčji potok garden centre and from one species from nature (common blueberry, Vaccinium myrtillus) for comparison. We stained the root samples with trypan blue and observed the degree of infection with ErM fungi under the microscope. We completed a survey of 8 species/hybrids and found quite rich infection with ErM in all plants, with some differences in colonisation rates. V. myrtillus was the most colonised, followed by Rhododendron impeditum, both also had very high colonisation with dark septate endophytes. Andromeda polyfolia had the lowest infection rate and no dark septate endophytes.

• Keywords: mycorrhiza, ornamental plants, Ericaceae, mycorrhizal infection