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Vpliv učinkovanja bakteriofagov na stafilokoke v različnih fizioloških stanjih
ID Krajnc, Doroteja (Author), ID Podgornik, Aleš (Mentor) More about this mentor... This link opens in a new window

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Abstract
Bakteriofagi so virusi, ki ne obstajajo in se ne morejo razmnoževati brez bakterijske gostiteljske celice. Ob tem je končni rezultat liza bakterijske celice, zato so prepoznani kot orodje v boju proti bakterijskim infekcijam. Pregled literature kaže na široko rabo bakteriofagov v prehrambeni industriji in kmetijstvu. Zaradi naraščujoče odpornosti bakterij na antibiotike je to potencialna rešitev tudi na področju medicine, natančneje pri uporabi na človeku. Zahteva po bakteriofagih zato narašča, posledično narašča potreba po optimizaciji proizvodnih procesov, razumevanje interakcije fag-bakterija pa je ključ načrtovanja le-teh. V tej luči so bili izvedeni eksperimenti na sistemu fag K- Staphylococcus capitis v šaržnih in kontinuirnih procesih, skozi katere smo opazovali latentno periodo, adsorpcijsko konstanto, pomnožitveno število in eklipsno periodo pri različnih specifičnih hitrostih rasti bakterijskih celic oziroma hitrostih redčenja. Fiziologija bakterijskih celic je namreč neposredno povezana z omenjenimi parametri. Pri naraščujoči specifični hitrosti rasti oziroma hitrosti redčenja smo v obeh sistemih opazili naračujočo adsorpcijsko konstanto ter pomnožitveno število. Latentna perioda in eklipsna perioda pri istem trendu specifične hitrosti rasti oziroma hitrosti redčenja padata v šaržnih sistemih, medtem ko za kontinuirne sisteme nismo pridobili zadostnih podatkov.

Language:Slovenian
Keywords:bakteriofagi, stafilokoki, šaržni sistem, kontinuirni sistem
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:FKKT - Faculty of Chemistry and Chemical Technology
Year:2023
PID:20.500.12556/RUL-149521 This link opens in a new window
COBISS.SI-ID:164001283 This link opens in a new window
Publication date in RUL:07.09.2023
Views:185
Downloads:15
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Secondary language

Language:English
Title:The effect of bacteriophages on Staphylococci in various physiological states
Abstract:
Bacteriophages are viruses that cannot exist and reproduce without a bacterial host cell. The end result is bacterial cell lysis, which means bacteriophages can be used as a fighting tool against bacterial infections. Literature shows the widespread use of bacteriophages in the food industry and agro-culture. The increasing bacterial resistance to antibiotics also makes it a potential solution in the field of medicine, more specifically human applications. Demand for bacteriophages is therefore increasing, and so is the need of optimal production processes. Understanding phage-bacteria interaction is key, therefore, experiments were carried out on the phage K- Staphylococcus capitis system in batch and continuous reactors. Since physiology of bacterial cells is directly linked to these parameters, latent period, adsorption constant, burst size and eclipse period were observed at different specific bacterial cell growth and dilution rates, respectively. At increasing specific growth rate and dilution rate, an increasing adsorption constant and burst size were observed in both systems. The decrease of latent period and eclipse period for the same trend in specific growth rate and dilution rate was observed in batch reactors, while for continuous reactors the data was insufficient.

Keywords:bacteriophages, staphylococci, batch reactors, continuous reactors

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