izpis_h1_title_alt

Virulentni dejavniki sevov Porphyromonas gingivalis, izoliranih iz posameznikov s parodontalno boleznijo
ID Bohar, Zala (Author), ID Skočaj, Matej (Mentor) More about this mentor... This link opens in a new window, ID Gašperšič, Rok (Co-mentor)

.pdfPDF - Presentation file, Download (1,73 MB)
MD5: D2DAFDDC827B3B06AECEA63227337A6F

Abstract
Parodontalna bolezen je kronična vnetna bolezen obzobnih tkiv in predstavlja najpogostejši razlog za izgubo zob v odrasli dobi. Nastanek kompleksnih zobnih oblog in prisotnost parodontalnih bakterij sta glavna razloga za razvoj bolezni. Agresivnejši potek bolezni pogosto povezujemo z viruletnimi dejavniki oz. virulenco bakterije P. gingivalis. Namen magistrske naloge je bil oblikovati specifične začetne oligonukleotide za nabor virulentnih dejavnikov P. gingivalis in z metodo PCR opredeliti njihovo prisotnost in prevalenco v kliničnih izolatih slovenskih pacientov s parodontalno boleznijo. V 53 kliničnih izolatih sevov P. gingivalis smo ugotovili visok delež genov za viruletne dejavnike, ki vplivajo na medceličnino, eritrocite in celično signalizacijo gostitelja. Pri agresivnejših oblikah parodontitisa smo zaznali razlike v prevalenci virulentnih dejavnikov za pritrjanje. Pacienti z napredovano parodontalno boleznijo imajo v največjem deležu prisotno gensko različico dolgih fimbrij fimA II (83 %) in v najmanjšem različico fimA V (3,8 %), med katerima smo potrdili tudi statistično značilno negativno korelacijo (p – vrednost < 0,001). V dokaj visokem deležu (60,4 %) smo v posameznih izolatih zaznali prisotnost različnih genskih zapisov dolgih fimbrij fimA, kar lahko najverjetneje pripišemo prisotnosti različnih sevom P. gingivalis v posameznem vzorcu. Sklenemo lahko, da dolge fimbrije FimA predstavljajo potencialno zanimivo področje nadaljnjih raziskav v povezavi z agresivnejšimi oblikami parodontalne bolezni, pri čemer uporaba metode PCR ni najbolj zanesljiva, zaradi možnosti lažnih pozitivnih rezultatov.

Language:Slovenian
Keywords:Porphyromonas gingivalis, parodontalna bolezen, genotipizacija, virulentne determinante
Work type:Master's thesis/paper
Typology:2.09 - Master's Thesis
Organization:BF - Biotechnical Faculty
Publisher:[Z. Bohar]
Year:2023
PID:20.500.12556/RUL-149078 This link opens in a new window
UDC:616.314-083(043.2)
COBISS.SI-ID:163241219 This link opens in a new window
Publication date in RUL:03.09.2023
Views:299
Downloads:65
Metadata:XML RDF-CHPDL DC-XML DC-RDF
:
Copy citation
Share:Bookmark and Share

Secondary language

Language:English
Title:Virulence determinants of Porphyromonas gingivalis strains isolated from individuals with periodontitis
Abstract:
Periodontal disease is a chronic inflammation of the periodontal tissues and is the most common cause of tooth loss in adulthood. The formation of complex dental plaque and the presence of periodontal bacteria are the main reasons for the development of the disease. A more aggressive course is often associated with bacterium P. gingivalis and it's virulence factors. The aim of our work was to design specific oligonucleotides (primers) for a set of virulence determinants and to identify their presence and prevalence in clinical isolates from Slovenian patients with periodontitis using the PCR method. In 53 clinical isolates, we determined a high percentage of virulence factors that affect the extracellular matrix, erythrocytes, and host cell signaling. The greatest differences in prevalence were observed in virulence factors for adhesion. The majority of patients with advanced periodontitis carried the genetic variant of long fimbriae fimA II (83 %) but only 3,8 % the fimA V variant, between which we also confirmed a statistically significant negative correlation (p-value < 0,001). In individual isolates we discovered a relatively high procentage (60, 4 %) of multiple genotypes of fimA, which can most likely be attributed to different strains of P. gingivalis in each sample. In conclusion, long fimbriae FimA represent a potentially interesting area for further research in relation to more aggressive forms of periodontal disease, with the use of the PCR method being unreliable due to the possibility of false positive results.

Keywords:Porphyromonas gingivalis, periodontitis, genotipization, virulent determinants

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Back