Anaphase-promoting complex subunit 1 associates with bone mineral density in human osteoporotic bone

Malavašič, Petra (avtor), Polajžer, Sara (avtor), Lovšin, Nika (avtor)

Genome-wide association studies (GWAS) are one of the most common approaches to identify genetic loci that are associated with bone mineral density (BMD). Such novel genetic loci represent new potential targets for the prevention and treatment of fragility fractures. GWAS have identified hundreds of associations with BMD; however, only a few have been functionally evaluated. A locus significantly associated with femoral neck BMD at the genome-wide level is intronic SNP rs17040773 located in the intronic region of the anaphase-promoting complex subunit 1 (ANAPC1) gene (p = 1.5 × 10$^-9$). Here, we functionally evaluate the role of ANAPC1 in bone remodelling by examining the expression of ANAPC1 in human bone and muscle tissues and during the osteogenic differentiation of human primary mesenchymal stem cells (MSCs). The expression of ANAPC1 was significantly decreased 2.3-fold in bone tissues and 6.2-fold in muscle tissue from osteoporotic patients as compared to the osteoarthritic and control tissues. Next, we show that the expression of ANAPC1 changes during the osteogenic differentiation process of human MSCs. Moreover, the silencing of ANAPC1 in human osteosarcoma (HOS) cells reduced RUNX2 expression, suggesting that ANAPC1 affects osteogenic differentiation through RUNX2. Altogether, our results indicate that ANAPC1 plays a role in bone physiology and in the development of osteoporosis.

• Jezik: Angleški jezik
• Ključne besede: anaphase-promoting complex subunit 1, osteoporosis, osteogenesis, osteoblast mineralisation, bone formation, bone mineral density
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 16 str.
• Številčenje: Vol. 24, no. 16, art. 12895
• PID: 20.500.12556/RUL-148657
• UDK: 616.71-007.234
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms241612895
• COBISS.SI-ID: 162450947
• Datum objave v RUL: 29.08.2023

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kompleksna podenota 1, ki spodbuja anafazo, osteogeneza, mineralizacija osteoblastov, tvorba kosti, mineralna gostota kosti, osteoporoza, kosti