The aim of this M. Sc Thesis was to determine whether dogs experience stress during animal-assisted interventions (AAA). 4 male and 3 female dogs of different ages and breeds participated in the study. The dogs were observed performing AAA for 4 consecutive working days, each at the same location. However, the locations varied between the dogs. During the work, the dogs behaviour, heart rate, and eye temperature were monitored. On the day the dog was not working, we took 5 heart rate measurements at home at rest and during calm walks. During the walk and work, the values of the parameters root mean square of successive differences of inter-beat intervals (RMSSD), standard deviation between the time intervals of consecutive heartbeats (SDNN), and the high frequency power (HF) were significantly lower and the values of the parameters heart rate (HR) and relative power in low frequency and the high frequency range (LF:HF) were higher than at rest. This result could be due to movement or a possible indicator of stress in the dog. There was no significant effect of the consecutive work day and work hour period (beginning, middle, end) on heart rate parameters during work, but there was significant decrease in the frequency of negative behaviours, suggesting that the dogs were not stressed during consecutive 5-minute periods of the work hour. There was no significant effect of the consecutive workday on the duration of each long-term behavioural pattern. Females exhibited negative behaviours significantly more often than males, who exhibited significantly more positive behaviours. Positive behaviours were recorded 3.68 ± 3.80 times in 5-minute periods of the working hour, whereas negative behaviours were recorded 2.98 ± 2.81 times. We found that monthly work frequency had no significant effect on heart rate parameters of the three types of activities (walking, working and resting), heart rate parameters at work, and frequency of expression of negative and positive behaviours. We also noted that the period of working hour had no significant effect on eye temperature. Based on the results of our study, we cannot confirm that the dogs were under stress during AAA.
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