In the master's thesis we studied the content of sugars, organic acids, vitamin C and phenolic compounds in fresh and frozen and stored fruits of highbush blueberry (Vaccinum corymbosum) at temperatures of -20 ° C and -80 ° C in two cultivars 'Bluecrop' and 'Duke'. Frozen fruits were stored up to 13 months and analyzed by HPLC at three different time points. We found that there are statistically significant differences between the contents of total sugars in the fruits of the 'Bluecrop' cultivar depending on the length of fruit storage. In the fruits of the 'Bluecrop' cultivar, there are statistically significant differences between the content of total acids in the interaction of the length of storage and storage condition of the fruits. Vitamin C content in the fruits of the 'Bluecrop' cultivar differ statistically significantly depending on the length of storage of the fruits. In the fruits of the 'Bluecrop' cultivar, there are statistically significant differences in the content of total phenolics depending on the length of fruit storage. We found that there are statistically significant differences between the content of total sugars in the fruits of 'Duke' cultivar depending on the length of fruit storage. In the fruits of 'Duke' cultivar, there are statistically significant differences between the content of total acids depending on the length of storage and storage conditions of the fruits, without the influence of their interaction. Vitamin C contents in the fruits of 'Duke' cultivar differ statistically significantly depending on the length of fruit storage. In the fruits of 'Duke' cultivar are statistically significant differences between the contents of total phenolics depending on the length of fruit storage. Fresh fruits were found to have the highest content of primary and secondary metabolites, which means that fresh fruits have higher content of health beneficial compounds.
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