Inactivation of the enveloped virus phi6 with hydrodynamic cavitation

Zupanc, Mojca (avtor), Zevnik, Jure (avtor), Filipić, Arijana (avtor), Gutiérrez-Aguirre, Ion (avtor), Ješelnik, Meta (avtor), Košir, Tamara (avtor), Ortar, Jernej (avtor), Dular, Matevž (avtor), Petkovšek, Martin (avtor)

The COVID-19 pandemic reminded us that we need better contingency plans to prevent the spread of infectious agents and the occurrence of epidemics or pandemics. Although the transmissibility of SARS-CoV-2 in water has not been confirmed, there are studies that have reported on the presence of infectious coronaviruses in water and wastewater samples. Since standard water treatments are not designed to eliminate viruses, it is of utmost importance to explore advanced treatment processes that can improve water treatment and help inactivate viruses when needed. This is the first study to investigate the effects of hydrodynamic cavitation on the inactivation of bacteriophage phi6, an enveloped virus used as a SARS-CoV-2 surrogate in many studies. In two series of experiments with increasing and constant sample temperature, virus reduction of up to 6.3 logs was achieved. Inactivation of phi6 at temperatures of 10 and 20 °C occurs predominantly by the mechanical effect of cavitation and results in a reduction of up to 4.5 logs. At 30 °C, the reduction increases to up to 6 logs, where the temperature-induced increased susceptibility of the viral lipid envelope makes the virus more prone to inactivation. Furthermore, the control experiments without cavitation showed that the increased temperature alone is not sufficient to cause inactivation, but that additional mechanical stress is still required. The RNA degradation results confirmed that virus inactivation was due to the disrupted lipid bilayer and not to RNA damage. Hydrodynamic cavitation, therefore, has the potential to inactivate current and potentially emerging enveloped pathogenic viruses in water at lower, environmentally relevant temperatures.

• Jezik: Angleški jezik
• Ključne besede: enveloped viruses, phi6, SARS-CoV-2, virus inactivation, hydrodynamic cavitation, water decontamination
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FS - Fakulteta za strojništvo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 8 str.
• Številčenje: Vol. 95, art. 106400
• PID: 20.500.12556/RUL-145264
• UDK: 532.528:578
• ISSN pri članku: 1350-4177
• DOI: 10.1016/j.ultsonch.2023.106400
• COBISS.SI-ID: 148619523
• Datum objave v RUL: 14.04.2023

Gradivo je del revije

• Naslov: Ultrasonics Sonochemistry
• Skrajšan naslov: Ultrason. sonochem.
• Založnik: Butterworth-Heinemann, Elsevier Science
• ISSN: 1350-4177
• COBISS.SI-ID: 707668

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: virusi z ovojnico, phi6, SARS-CoV-2, inaktivacija virusov, hidrodinamična kavitacija, dekontaminacija vode