Periodontal diseases are classified as the most common chronical diseases of nowdays. Among them, the most common are gingivits and periodontitis. Gingivitis is a mild inflammatory disease of gingiva, which can develop into a more severe form named periodontitis. Latter is known for deep inflammation of the tissue, emergence of gingival pockets, decomposition of bone tissue and finally loss of teeth. Inflammation and tissue breakdown is mainly caused by periodontal pathogens which mostly belong to Gram negative bacteria. They produce biofilms which are the main contributors for developement and progression of the disease. The membranes of these pathogens contain a unique sphingolipd ceramid phosphoethanolamine (CPE), which could be a potential biomarker for assesing the developement of periodontal diseases over time. Aegerolysins are unique proteins, that have the abillity to bind membrane lipids, particularly the CPE. Ceramide phosphoethanolamine could represents a great periodontal diseases-associated biomarker, since is scarsly present in mammalian cells. In the thesis we have focused on development of a method for measuring the quantity of CPE in lipid membranes of multilamelar vesicles in order to set a new diagnostic tool for periodontal diseases. The test is based on measuring the level of fluorescence of fluorescently tagged egerolysin EryA-mCherry, which binds irrevesibly to CPE in membranes of lipid vesicles when combined with its protein partner, pleurotolysin B.
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