Head and neck squamous cell carcinomas (HNSCCs) are common cancers with high mortality, that can be caused by human papilloma virus (HPV). In HNSCC, HPV infections can be divided into productive and non-productive infection. In non-productive infection, the viral genome is incorporated into the host genome, which leads to malignant transformation of cells due to the uncontrolled expression of oncoproteins E6 and E7. Oncoproteins E6 and E7 affect the proteins p53 and pRB, which are responsible for regulating the cell cycle and triggering apoptosis. We prepared HPV-positive murine HNSCC MOC1 cell line by viral transduction of genes for oncoproteins E6 and E7, and evaluated the established cell lines, based on expression of proteins E6 and E7 at mRNA and protein levels. RNA was isolated from the cell lines, it's concentration and purity determined, and then RNA was used to analyze the expression of E6 and E7 proteins, relative to the housekeeping genes for beta-actin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). We also prepared fixed cell samples, which were immunofluorescently stained with antibodies against proteins E6 and E7, thus determining expression of E6 and E7 at the protein level. We have demonstrated that expression across passages was stable at both levels.
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