Peripheral blood lymphocyte subtypes in dogs with different stages of myxomatous mitral valve disease

Druzhaeva, Natalia (avtor), Nemec Svete, Alenka (avtor), Ihan, Alojz (avtor), Pohar, Katka (avtor), Domanjko-Petrič, Aleksandra (avtor)

Background: Data on alterations in peripheral blood lymphocyte (PBL) subtypes in dogs with myxomatous mitral valve disease (MMVD) is lacking. Objectives: To investigate PBL subtypes and their correlation with parameters of inflammation and MMVD progression markers in dogs with different stages of MMVD. Animals: Seventy-eight client-owned dogs: 65 with MMVD (American College of Veterinary Internal Medicine [ACVIM] classification stages B2, C, and D) and 13 healthy controls. Methods: Prospective cross-sectional study. Complete cardiac assessment, flow cytometry (T lymphocytes [CD3+], their subtypes [CD3+CD4+, CD3+CD8+, CD3+CD4+CD8+, CD3+CD4−CD8−], and B lymphocytes [CD45+CD21+]) and measurement of N-terminal pro B-type natriuretic peptide, cardiac troponin I, and C-reactive protein concentrations were performed. Results: The percentage of CD3+CD4+ lymphocytes was significantly lower in stable ACVIM C patients (P = .01) and unstable ACVIM C and D patients (P = .003), the percentage of CD3+CD8+ lymphocytes was significantly higher in stable ACVIM C patients (P = .01) and unstable ACVIM C and D patients (P = .01), CD3+CD8+ lymphocyte concentration was significantly higher in unstable ACVIM C and D patients (P = .05), and the CD3+CD4+/CD3+CD8+ ratio was significantly lower in stable ACVIM C patients (P = .01) and unstable ACVIM C and D patients (P = .01) compared with healthy controls. Conclusions and Clinical Importance: The percentages of CD3+CD4+ and CD3+CD8+ PBL and CD4+/CD8+ ratio were altered in MMVD dogs with congestive heart failure (ACVIM C, D), but not in ACVIM B2, suggesting involvement of these PBL subtypes in the pathogenesis of congestive heart failure in dogs with MMVD.

• Jezik: Angleški jezik
• Ključne besede: canine, CD21 B lymphocytes, CD3 T lymphocytes, CD4 T helper lymphocytes, CD45, CD8 cytotoxic T lymphocytes, congestive heart failure, degenerative mitral valve disease, flow cytometry, heart diseases, dogs
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: Str. 2112-2122
• Številčenje: Vol. 35, iss. 5
• PID: 20.500.12556/RUL-144752
• UDK: 636.7.09:616.1
• ISSN pri članku: 1939-1676
• DOI: 10.1111/jvim.16207
• COBISS.SI-ID: 69808131
• Datum objave v RUL: 10.03.2023

Gradivo je del revije

• Naslov: Journal of veterinary internal medicine
• Založnik: American College of Veterinary Internal Medicine, Wiley
• ISSN: 1939-1676
• COBISS.SI-ID: 517778713

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0053
• Naslov: Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih