Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathon

Jan, Zala (author), Drab, Mitja (author), Drobne, Damjana (author), Bedina Zavec, Apolonija (author), Benčina, Mojca (author), Drašler, Barbara (author), Hočevar, Matej (author), Krek, Judita Lea (author), Pađen, Ljubiša (author), Pajnič, Manca (author), Repar, Neža (author), Šimunič, Boštjan (author), Štukelj, Roman (author), Kralj-Iglič, Veronika (author)

Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and >15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs’ concentration in isolates from blood (46%; p0.8; p<0.05), in ChE activity in whole blood (47%; p<0.001), in plasma (34%; p<0.01), and in erythrocyte suspension (54%; p<0.001), as well as in GST activity in erythrocyte suspension (16%; p<0.01) and in IL-6 concentration in plasma (63%; p<0.05). We found no change in GST activity in plasma and in TNF-α concentration in plasma. Correlations (>0.8; p<0.001) between CNVs’ concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs’ concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs’ concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.

• Language: English
• Keywords: membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences; FE - Faculty of Electrical Engineering; BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: Str. 443-456
• Numbering: Vol. 16
• PID: 20.500.12556/RUL-144718
• UDC: 577.1:796.422.16
• ISSN on article: 1178-2013
• DOI: 10.2147/IJN.S282200
• COBISS.SI-ID: 48113411
• Publication date in RUL: 09.03.2023

Record is a part of a journal

• Title: International journal of nanomedicine
• Publisher: Dove Medical Press
• ISSN: 1178-2013
• COBISS.SI-ID: 3223828

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• License: CC BY-NC 3.0, Creative Commons Attribution-NonCommercial 3.0 Unported
• Link: http://creativecommons.org/licenses/by-nc/3.0/
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Secondary language

• Language: Slovenian
• Keywords: vezikulacija membrane, fizični napor, krvni vzorci, vnetni procesi, celični nanovezikli, maraton