A dual biomarker TK1 protein and CA125 or HE4-based algorithm as a better diagnostic tool than ROMA Index in early detection of ovarian cancer

Cviič, Diana (author), Jagarlamudi, K. K. (author), Meglič, Leon (author), Škof, Erik (author), Zore, Andrej (author), Lukanovič, David (author), Eriksson, Staffan (author), Osredkar, Joško (author)

Background: The early detection of ovarian cancer is presently not effective, and it is crucial to establish biomarkers for the early diagnosis of ovarian cancer to improve the survival of patients. Materials and methods: The aim of this study was to investigate the role of thymidine kinase 1 (TK1) in combination with CA 125 or HE4 to serve as a potential diagnostic biomarkers for ovarian cancer. In this study, a set of 198 serum samples consisting of 134 ovarian tumor patients and 64 healthy age-matched controls were analyzed. The TK1 protein levels in serum samples were determined using the AroCell TK 210 ELISA. Results: A combination of TK1 protein with CA 125 or HE4 showed better performance than either of them alone in the differentiation of early stage ovarian cancer from the healthy control group, but also a significantly better performance than the ROMA index. However, this was not observed using a TK1 activity test in combination with the other markers. Furthermore, the combination of TK1 protein and CA 125 or HE4 could differentiate early stage disease (stage I, II) more efficiently from advanced-stage (stage III, IV) disease (p < 0.0001). Conclusions: The combination of TK1 protein with CA 125 or HE4 increased the potential of detecting ovarian cancer at early stages.

• Language: English
• Keywords: thymidine kinase 1, TK 210 ELISA, TK-liaison, CA 125, HE4, ROMA index, ovarian cancer
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy; MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 18 str.
• Numbering: Vol. 15, iss. 5, art. 1593
• PID: 20.500.12556/RUL-144644
• UDC: 612.621:616-006
• ISSN on article: 2072-6694
• DOI: 10.3390/cancers15051593
• COBISS.SI-ID: 144061955
• Publication date in RUL: 06.03.2023

Record is a part of a journal

• Title: Cancers
• Shortened title: Cancers
• Publisher: MDPI
• ISSN: 2072-6694
• COBISS.SI-ID: 517914137

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: timidin kinaza 1, indeks ROMA