Characterization of the Slovene autochthonous rose hybrid Rosa pendulina × spinosissima (Rosa reversa Waldst. and Kit) using biochemical patterns of the plant blossoms

Kunc, Nina (author), Hudina, Metka (author), Bavcon, Jože (author), Vreš, Branko (author), Luthar, Zlata (author), Gostinčar, Kristina (author), Mikulič Petkovšek, Maja (author), Osterc, Gregor (author), Ravnjak, Blanka (author)

The Rosa genus is characterized by great variability and, consequently, they easily hybridize. The petals of R. pendulina, R. spinosissima and their hybrid Rosa pendulina x spinosissima, collected in western Slovenia, were included in the research. We performed morphometric analysis using keys to determine roses and genetic analysis to determine the genome size. The phenolic compound content in petals of all rose flowers was measured by liquid chromatography and mass spectrometry (HPLC-MS). Using flow cytometry, we could confirm the native hybridization process due to the amount of 2C DNA. The value of R. pendulina was 1.71 pg, of R. spinosissima 1.60 pg and of the hybrid 1.62 pg. The value for the hybrid was close to values measured for parent plants and, at the same time, those values of parent plants significantly differed from each other. Our results showed that the content of phenolic compounds in petals decreased after crossing. We found that the highest total phenolic content (178.9 g/kg FW) was measured in R. spinossisima, the lowest content was analyzed for the hybrid (84.36 g/kg FW) and the content for R. pendulina was between these two values (110.58 g/kg FW). The content of flavanols and flavonols was lowest in the hybrid petals, whereas the content of anthocyanins was highest in the hybrid petals.

• Language: English
• Keywords: autochthonous rose, HPLC-MS, flow cytometry, hybrids, phenols, Rosa pendulina, Rosa spinosissima, Rosa pendulina x spinosissima
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 16 str.
• Numbering: Vol. 12, iss. 3, art. 505
• PID: 20.500.12556/RUL-143958
• UDC: 665.5
• ISSN on article: 2223-7747
• DOI: 10.3390/plants12030505
• COBISS.SI-ID: 139079171
• Publication date in RUL: 24.01.2023

Record is a part of a journal

• Title: Plants
• Shortened title: Plants
• Publisher: MDPI
• ISSN: 2223-7747
• COBISS.SI-ID: 523345433

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: domača vrnica, pretočna citometrija, fenoli

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0013
• Name: Hortikultura

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0077
• Name: Kmetijske rastline - genetika in sodobne tehnologije