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Rhodiola rosea reduces intercellular signaling in Campylobacter jejuni
ID Kunčič, Ajda (Author), ID Bucar, Franz (Author), ID Smole Možina, Sonja (Author)

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Abstract
Campylobacter jejuni is a major foodborne pathogen and the leading cause of bacterial gastroenteritis, i.e., campylobacteriosis. Besides searching for novel antimicrobials, identification of new targets for their action is becoming increasingly important. Rhodiola rosea has long been used in traditional medicine. Ethanolic extracts from the roots and rhizomes of the plant contain a wide range of bioactive compounds with various pharmacological activities. In this study, cultivated plant materials have been used, i.e., “Mattmark” and “Rosavine”. Through optimized protocols, we obtained fractions of the initial ethanolic extracts rich in most important bioactive compounds from R. rosea, including salidroside, rosavins, proanthocyanidins (PACs), and flavonoids. The antimicrobial activity in relation to the chemical composition of the extracts and their fractions was studied with an emphasis on C. jejuni AI-2-mediated intercellular signaling. At concentration 15.625 mg/L, bioluminescence reduction rates varied from 27% to 72%, and the membrane remained intact. Fractions rich in PACs had the strongest antimicrobial effect against C. jejuni, with the lowest minimal inhibitory concentrations (MICs) (M F3 40%: 62.5 mg/L; R F3 40%: 250 mg/L) and the highest intercellular signaling reduction rates (M F3 40%: 72%; R F3 40%: 65%). On the other hand, fractions without PACs were less effective (MICs: M F5 PVP: 250 mg/L; R F5 PVP: 1000 mg/L and bioluminescence reduction rates: M F5 PVP: 27%; R F5 PVP: 43%). Additionally, fractions rich in flavonoids had strong antimicrobial activity (MICs: M F4 70%: 125 mg/L; R F4 70%: 250 mg/L and bioluminescence reduction rates: M F4 70%: 68%; R F4 70%: 50%). We conclude that PACs and flavonoids are crucial compound groups responsible for the antimicrobial activity of R. rosea roots and rhizomes in C. jejuni.

Language:English
Keywords:Rhodiola rosea, proanthocyanidins, flavonoids, Campylobacter jejuni, LuxS, intercellular signaling
Work type:Article
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publication status:Published
Publication version:Version of Record
Year:2022
Number of pages:13 str.
Numbering:Vol. 11, iss. 9, art. 1220
PID:20.500.12556/RUL-142445 This link opens in a new window
UDC:579.25:547.9:615.33
ISSN on article:2079-6382
DOI:10.3390/antibiotics11091220 This link opens in a new window
COBISS.SI-ID:121236483 This link opens in a new window
Publication date in RUL:09.11.2022
Views:704
Downloads:147
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Record is a part of a journal

Title:Antibiotics
Shortened title:Antibiotics
Publisher:MDPI
ISSN:2079-6382
COBISS.SI-ID:522975769 This link opens in a new window

Licences

License:CC BY 4.0, Creative Commons Attribution 4.0 International
Link:http://creativecommons.org/licenses/by/4.0/
Description:This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

Language:Slovenian
Keywords:rožni koren, bioaktivne snovi, protimikrobne snovi, patogeni mikroorganizmi

Projects

Funder:ARRS - Slovenian Research Agency
Project number:P4-0116
Name:Mikrobiologija in biotehnologija živil in okolja

Funder:ARRS - Slovenian Research Agency
Project number:J4-2542
Name:Mikrobne interakcije kot temelj biokontrole bakterij Campylobacter jejuni

Funder:EC - European Commission
Funding programme:Erasmus+

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