This master's thesis describes the synthesis of peptides on a solid support using the Fmoc/tBu synthesis strategy. The synthesis was carried out manually using a syringe equipped with a frit, where the reaction conditions were established, which were then transferred to the flow reactor. Using manual SPPS and flow SPPS, we synthesized 3 different peptides. The peptide H-LSPPRYP-OH and peptide H-GLTFDEIQGLTYLQV- OH were synthesised by using manual SPPS, and peptides H-LSPPRYP-OH and H- TIEQDYILILAVMHCSREPGYWKSRR-OH were synthesized by using flow SPPS. With the H-LSPPRYP-OH peptide synthesis, we studied the differences between the use of manual and flow synthesis of peptides on a solid support. The peptide H- TIEQDYILILAVMHCSREPGYWKSRR-OH was synthesized as one of the peptide fragments, with the aim of further synthesis of a 96 amino acid long protein using serine/threonine chemical ligation. All synthesized peptides were characterized by HRMS and HPLC analysis. We found that flow SPPS is much faster than manual SPPS, however resulted products in lower yield and purity.
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