Grape seed oil is a byproduct from wine industry and is becoming increasingly popular. In this master thesis we investigated fatty acid composition, free fatty acid, peroxide value and antioxidative potential of grape seed oils. Alltogether, 23 samples of grape seed oil were analysed, of which 6 were produced from white grape varieties, 11 were produced from red grape varieties while 6 oil samples were of unknown variety and were bought on market. The average content off free fatty acids in oils of white varieties amounted to 1.01 %, for 0.98 % for red varieties and 0.28 % oils of unknown origin, all values were below recommended treshold od 2%. The average peroxide values were above recommended value 7 mmol/kg, 56.8 for white, 59.31 for red and 18,78 for oils of unknown variety. Oils contain from 62.7 to 71,9 % polyunsaturated fatty acids (PUFA), from 16.2 to 26.3 % monounsatured fatty acids (MUFA) and from 11 to 11.9 % saturated fatty acids (SFA). Significant differences between studied oil groups were found for linolic and oleic acid, for PUFA, MUFA and SFA. Linoleic acid was the main fatty acid followed by oleic and palmitic acid. Studied oils also contain minor amounts of palmitic acid, alpha linolenic acid content of both amounts to 0,5%.
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