Enrichment of plasma in platelets and extracellular vesicles by the counterflow to erythrocyte settling

Božič, Darja (avtor), Vozel, Domen (avtor), Hočevar, Matej (avtor), Jeran, Marko (avtor), Jan, Zala (avtor), Pajnič, Manca (avtor), Pađen, Ljubiša (avtor), Iglič, Aleš (avtor), Battelino, Saba (avtor), Kralj-Iglič, Veronika (avtor)

In order to prepare optimal platelet and extracellular vesicle (EV)-rich plasma for the treatment of chronic temporal bone inflammation, we studied effects of centrifugation parameters on redistribution of blood constituents in blood samples of 23 patients and 20 volunteers with no record of disease. Concentrations of blood cells and EVs were measured by flow cytometry. Sample content was inspected by scanning electron microscopy. A mathematical model was constructed to interpret the experimental results. The observed enrichment of plasma in platelets and EVs after a single spin of blood depended on the erythrocyte sedimentation rate, thereby indicating the presence of a flow of plasma that carried platelets and EVs in the direction opposite to settling of erythrocytes. Prolonged handling time correlated with the decrease of concentration of platelets and larger EVs in platelet and EV-rich plasma (PVRP), R = −0.538, p = 0.003, indicating cell fragmentation during the processing of samples. In further centrifugation of the obtained plasma, platelet and EV enrichment depended on the average distance of the sample from the centrifuge rotor axis. Based on the agreement of the model predictions with observations, we propose the centrifugation protocol optimal for platelet and EV enrichment and recovery in an individual sample, adjusted to the dimensions of the centrifuge rotor, volume of blood and erythrocyte sedimentation rate.

• Jezik: Angleški jezik
• Ključne besede: cellular vesicles, extracellular vesicles, microvesicles, platelet microparticles, platelet rich plasma, wound healing
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: ZF - Zdravstvena fakulteta ; FE - Fakulteta za elektrotehniko; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: Str. 592-602
• Številčenje: Vol. 33, iss. 4
• PID: 20.500.12556/RUL-141254
• UDK: 616
• ISSN pri članku: 0953-7104
• DOI: 10.1080/09537104.2021.1961716
• COBISS.SI-ID: 74092547
• Datum objave v RUL: 27.09.2022

Gradivo je del revije

• Naslov: Platelets
• Skrajšan naslov: Platelets
• Založnik: Taylor & Francis
• ISSN: 0953-7104
• COBISS.SI-ID: 826901

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.