Histološka analiza poškodb na listih krompirja, okuženega z virusom Y krompirja

Žuran, Ana (author), Dolenc Koce, Jasna (mentor), Kladnik, Aleš (comentor)

Virus YNTN krompirja na listih občutljivih rastlin in rastlin, ki se na okužbo odzovejo s preobčutljivostnim odzivom povzroča nekrotične lezije. V nalogi smo z uporabo različnih histoloških tehnik v genetsko različnih rastlinah krompirja (Solanum tuberosum L.) sorte Rywal in NahG-Rywal analizirali lezije na nivoju tkiv in celic, izmerili njihovo velikost, lokalizirali pojavljanje programirane celične smrti in nalaganje fenolnih kislin ter flavonoidov. Rezultati meritev premera lezij na prečnih prerezih listov so pokazali, da se na listih rastlin NahG-Rywal razvijejo značilno večje lezije kot na rastlinah sorte Rywal. Dodatne meritve površine lezij na svežih preparatih listov so pokazale manjšo razliko med genotipoma, vendar le-ta ni bila statistično značilna. Z reakcijo TUNEL smo v nekrotičnih celicah in v okolici lezije na listih rastlin NahG-Rywal zaznali fragmentirano DNA v jedrih, ki bi lahko bila posledica programirane celične smrti. Na podlagi spremembe avtofluorescence v alkalnem mediju smo na območju lezije ugotovili prisotnost fenolnih kislin, kot je npr. ferulična kislina. Prisotnost flavonoidov smo potrdili v celicah okoli lezij na svežih preparatih okuženih listov, medtem ko je bilo njihovo barvanje na fiksiranem tkivu neuspešno.

• Language: Slovenian
• Keywords: krompir, virus Y krompirja, preobčutljivostni odziv, lezije, programirana celična smrt, flavonoidi, histološka analiza
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Place of publishing: Ljubljana
• Publisher: [A. Žuran]
• Year: 2022
• PID: 20.500.12556/RUL-141066
• UDC: 632
• COBISS.SI-ID: 123497731
• Publication date in RUL: 23.09.2022

ŽURAN, Ana, 2022, Histološka analiza poškodb na listih krompirja, okuženega z virusom Y krompirja [online]. Master’s thesis. Ljubljana : A. Žuran. [Accessed 25 April 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=141066

Secondary language

• Language: English
• Title: Histological analysis of lesions on potato leaves infected with potato virus Y

Abstract

Potato virus YNTN causes necrotic lesions in leaves of susceptible plants and plants reacting to infection with a hypersensitive response. In this study, we used different histological techniques to analyse the lesions at the tissue and cell level, measured their size, localised the occurrence of programmed cell death and the accumulation of phenolic acids and flavonoids in leaves of genetically different potato plants (Solanum tuberosum L.) cv. Rywal and NahG-Rywal. Lesion diameter measurements on leaf cross-sections showed that NahG-Rywal plants develop significantly larger lesions than cv. Rywal plants. Additional measurements of the lesion area on fresh leaf preparations showed a slight difference between the two genotypes, but this was not statistically significant. Using TUNEL reaction we detected fragmented DNA in the nuclei of necrotic cells and in the vicinity of the lesion in leaves of NahG-Rywal plants, which could be the result of programmed cell death. The presence of phenolic compounds, such as ferulic acid, in the lesion area was detected by autofluorescence changes in alkaline medium. The presence of flavonoids was confirmed in the cells around the lesions in fresh preparations of infected leaves, whereas their staining on fixed tissue was unsuccessful.

• Keywords: potato, potato virus Y, hypersensitive response, lesions, programmed cell death, flavonoids, histological analysis