Določevanje artemizinina v miceliziranih formulacijah

Šijanec, Ana (author), Pompe, Matevž (mentor)

Artemizinin je zdravilna, protimalarijska učinkovina, naravno pridobljena iz sladkega pelina (Artemisia annua). Od odkritja leta 1972, se je artemizinin uporabljal kot zdravilo proti malariji, v zadnjih letih pa so ga zaradi njegove dokazane vsestranskosti začeli vse bolj uporabljati v farmaciji in prehrambeni industriji. Je zelo slabo topen v številnih topilih, zato ga je v večino pripravkov potrebno uvajati v micelarnih formulacijah. Za doseganje učinkovitosti in varnosti izdelkov, je potrebna ustrezna analiza le-teh. V diplomskem delu sem se ukvarjala z razvojem metode za določevanje artemizinina v micelarnih formulacijah, ki, v kolikor mi je znano, še nikoli ni bila objavljena. Sprva sem razvila in validirala metodo za določevanje artemizinina na HPLC-UV/VIS. Zatem sem preverjala vpliv temperature in antioksidanta na analit in na koncu razvila metodo za določevanje artemizinina v micelarnih formulacijah.

• Language: Slovenian
• Keywords: artemizinin, vitamin C, HPLC-UV/VIS, ekstrakcija
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2022
• PID: 20.500.12556/RUL-140025
• COBISS.SI-ID: 129794563
• Publication date in RUL: 09.09.2022

Secondary language

• Language: English
• Title: Determination of artemisinin in micellized formulations

Abstract

Artemisinin is a medicinal, antimalarial substance, naturally derived from sweet wormwood (Artemisia annua). Since 1972, artemisinin has been used as an antimalarian drug. Its proven versatility in recent years has led to its increased use in the pharmaceutical and food industry. Due to its poor solubility in many solvents, micellar formulation is commonly used as a drug delivery system. To achieve product safety and efficiency, products need to be properly analysed. In this Bachelor's thesis I worked on the development of a method for the determination of artemisinin in micellar formulations, which, to my knowledge, has never been published before. Initially, I developed a method for the determination of artemisinin by HPLC-UV/VIS. Then I tested the effect of temperature and the presence of an antioxidant on the analyte and developed a method for the determination of artemisinin in micellar formulations.

• Keywords: artemisinin, vitamin C, HPLC-UV/VIS, extraction