Circular RNAs regulate many processes in cells and participate in the development of diseases. Many have altered expression in cancer cells and their role in cancer development, especially hepatocellular carcinoma (HCC), is studied in cell models. The aim of the work was the preparation of lentiviral vectors for the overexpression and silenced expression of circular RNAs. As candidates for vector cloning, we selected exosomal circular RNAs hsa_circ_0001861 and hsa_circ_0070039, which are downregulated in liver cancer. We could not determine silenced expression, as we found that siRNAs also bind to other target mRNAs. As a result, other genes would also be silenced due to excessive non-specificity. Using molecular cloning methods such as PCR amplification, restriction, ligation and transformation, we managed to insert the desired sequence hsa_circ_0001861 into the plasmid vector pRRcirc, which we also proved by determining the nucleotide sequence by Sanger method. Because circRNAs have a very short sequence specific to them, their detection, amplification and silencing are often limited, which makes research of their function in cell lines impossible.
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