Your browser does not allow JavaScript!
JavaScript is necessary for the proper functioning of this website. Please enable JavaScript or use a modern browser.
Open Science Slovenia
Open Science
DiKUL
slv
|
eng
Search
Browse
New in RUL
About RUL
In numbers
Help
Sign in
Immunocastration in adult boars as a model for late-onset hypogonadism
ID
Batorek Lukač, Nina
(
Author
),
ID
Kress, Kevin
(
Author
),
ID
Čandek-Potokar, Marjeta
(
Author
),
ID
Fazarinc, Gregor
(
Author
),
ID
Škrlep, Martin
(
Author
),
ID
Poklukar Žnidaršič, Klavdija
(
Author
),
ID
Wesoly, Raffael
(
Author
),
ID
Stefanski, Volker
(
Author
),
ID
Vrecl, Milka
(
Author
)
PDF - Presentation file,
Download
(4,04 MB)
MD5: E4EFCC706BA22DD6CE8764D76A825E7D
URL - Source URL, Visit
https://onlinelibrary.wiley.com/doi/10.1111/andr.13219
Image galllery
Abstract
Background: While immunocastration has been studied in male pre-pubertal pigs, data on older, sexually mature animals are limited. To understand the physiological effects of androgen deprivation in the late sexual development phase, we compared mature immunocastrated boars (n = 19; average age = 480 days) to young male immunocastrated pigs (n = 6; average age = 183 days) and young entire males (n = 6; average age = 186 days) as positive and negative controls, respectively. Objectives: We hypothesized that the timing of gonadotropin-releasing hormone suppression (early or late sexual development phases) influences the extent of reproductive function inhibition, histological structure of testicular tissue, and expression levels of selected genes related to steroid metabolism. Matherial and methods: Antibody titer, hormonal status, and histomorphometric analysis of testicular tissue were subjected to principal component analysis followed by hierarchical clustering to evaluate the immunocastration effectiveness in mature boars. Results: Hierarchical clustering differentiated mature immunocastrated boars clustered with young immunocastrated pigs from those clustered with entire males. Although all mature immunocastrated boars responded to vaccination, as evidenced by the increased gonadotropin-releasing hormone antibody titers (p < 0.001), decreased serum luteinizing hormone concentrations (p = 0.002), and changes in testicular tissue vascularization (lighter and less red testicular parenchyma; p ≤ 0.001), the responses were variable. Sharp decreases in testes index (p < 0.001), Leydig cell volume density (p < 0.001), Leydig cell nucleus-to-cytoplasm ratio (p < 0.001), and testosterone concentration (p < 0.001) were observed in mature immunocastrated boars clustered with young immunocastrated pigs compared with those that clustered with entire males. Additionally, mature immunocastrated boars clustered with young immunocastrated pigs showed lower hydroxysteroid 17-beta dehydrogenase 7 expression than entire males (p < 0.05). The young immunocastrated pigs group showed higher follicle-stimulating hormone receptors than the entire males and mature immunocastrated boars, lower steroidogenic acute regulatory protein expression levels compared with entire males, and mature immunocastrated boars clustered with entire males (p < 0.01). Conclusion: The two-dose vaccination regime resulted in progressive but variable regression of testicular function in adult (post-pubertal) pigs; however, it was insufficient to induce a complete immunocastration response in all animals.
Language:
English
Keywords:
response to immunocastration
,
pigs
,
anti-GnRH
,
morphometry
,
testicular mRNA expression
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
VF - Veterinary Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2022
Number of pages:
Str. 1217-1232
Numbering:
Vol. 10, iss. 6
PID:
20.500.12556/RUL-139580
UDC:
636.4
ISSN on article:
2047-2927
DOI:
10.1111/andr.13219
COBISS.SI-ID:
112842755
Publication date in RUL:
05.09.2022
Views:
699
Downloads:
326
Metadata:
Cite this work
Plain text
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Copy citation
Share:
Record is a part of a journal
Title:
Andrology
Shortened title:
Andrology
Publisher:
Wiley, American Society of Andrology, European Academy of Andrology
ISSN:
2047-2927
COBISS.SI-ID:
519114521
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
Z7-9416
Name:
Imunokastracija kot metoda deprivacije androgenov in njeni učinki na fiziološki odziv v zgodnji in pozni fazi spolnega razvoja prašičev
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0133
Name:
Trajnostno kmetijstvo
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0053
Name:
Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih
Funder:
EC - European Commission
Funding programme:
H2020
Project number:
696231
Name:
European Research Area on Sustainable Animal Production Systems
Acronym:
SusAn
Funder:
EC - European Commission
Funding programme:
ERA Net SusAn
Project number:
631-10/2015/7
Name:
Sustainability in pork production with immunocastration
Acronym:
SuSi
Funder:
Other - Other funder or multiple funders
Funding programme:
COST
Project number:
CA 15215
Name:
Innovative Approaches for Pork Production with Entire Males
Acronym:
IPEMA
Similar documents
Similar works from RUL:
Similar works from other Slovenian collections:
Back