izpis_h1_title_alt

Identifikacija in karakterizacija biotehnološko zanimivih morskih mikroorganizmov za razgradnjo agarja
ID Rebselj, Jure (Author), ID Accetto, Tomaž (Mentor) More about this mentor... This link opens in a new window

.pdfPDF - Presentation file, Download (1,51 MB)
MD5: 3111434139597150B2957A07F28CE950

Abstract
Agar je polisaharid iz celičnih sten rdečih alg, ki ima skupaj z razgrajenimi produkti velik pomen v industriji in farmaciji. Hidrolizo agarja je mogoče izvesti kemično ali encimatsko, pri čemer je slednja bolj ekonomsko in ekološko primerna. Cilj prvega dela moje diplomske naloge je bil izolirati mikroorganizme sposobne hidrolize agarja iz vzorca morske vode z gojenjem na gojiščih z agarjem kot primarnim virom hranil. Izbranim mikroorganizmom sem določil rod z izolacijo in sekvenciranjem 16S gena. Za določitev optimalnih pogojev za kultivacijo ter produkcijo encima sem mikroorganizme gojil na trdnih in tekočih gojiščih z različno sestavo ter pri različnih temperaturah. Sevi, ki so pokazali najboljšo encimsko aktivnost in s tem največji biotehnološki potencial, so predstavniki rodov Tamlana, Saccharophagus in Microbulbifer. V drugem delu naloge sem z izbranih sevov izoliral proizvedeno mešanico encimov za določitev encimske aktivnosti pri različnih temperaturah s kvalitativnim testom ter določitev velikostnega reda agaraz z encimogramom. Rezultati so pokazali, da imajo encimi najboljšo aktivnost pri 37°C, nekateri pa dobro delujejo tudi pri nižjih temperaturah. Izbrane bakterije so biotehnološko zanimive, a neprimerne za uporabo v industriji zaradi časa rasti in potrebe po specifičnem gojišču. Njihovi encimi pa so zanimivi za nadaljnje raziskave in modifikacije za industrijsko uporabo.

Language:Slovenian
Keywords:agar, agaraza, hidroliza agarja, morski mikroorganizmi, Microbulbifer, Saccharophagus, Tamlana
Work type:Bachelor thesis/paper
Typology:2.11 - Undergraduate Thesis
Organization:BF - Biotechnical Faculty
Year:2022
PID:20.500.12556/RUL-139492 This link opens in a new window
COBISS.SI-ID:124985091 This link opens in a new window
Publication date in RUL:03.09.2022
Views:614
Downloads:42
Metadata:XML DC-XML DC-RDF
:
Copy citation
Share:Bookmark and Share

Secondary language

Language:English
Title:Identification and characterization of biotechnologically interesting marine microorganisms for degradation of agar
Abstract:
Agar is a polysaccharide from red algae’s cell wall, which has together with its hydrolysis products considerable importance in industry and pharmacy. Hydrolysis is possible chemically or enzymatically, which is more economically and ecologically suitable. The goal of the first part of my thesis was the isolation of agar degrading microorganisms from a sample of seawater, by cultivation on media with agar as a primary source of nourishments. DNA was isolated from selected microorganisms to determine their genus with 16S sequencing. To determine their optimal cultivation and enzyme production parameters, microorganisms were cultivated on different solid and liquid media at different temperatures. Strains that had shown the best enzymatic activity and biotechnical potential were Tamlana, Saccharophagus and Microbulbifer. In the second part of the thesis, enzyme mixture from each strain was isolated to determine enzymatic activity at different temperatures with a qualitative test and to determine a mass class with a zymogram. The results had shown that the optimal temperature for enzymes' activity is thirty-seven degrees Celsius however, some still had good activity at lower temperatures. Isolated marine bacteria are biotechnologically interesting but unsuitable to use in the industry because of their longer growth time and need for a marine medium. On the other hand, their enzymes are interesting for future research and modifications for potential industrial usage.

Keywords:agar, agarase, agar hydrolysis, marine microorganisms, Microbulbifer, Saccharophagus, Tamlana

Similar documents

Similar works from RUL:
Similar works from other Slovenian collections:

Back