Cytosine methylation in genomic DNA and characterization of DNA methylases and demethylases and their expression profiles in viroid-infected hop plants (Humulus lupulus var. ‘Celeia’)

Sečnik, Andrej (avtor), Štajner, Nataša (avtor), Radišek, Sebastjan (avtor), Kunej, Urban (avtor), Križman, Mitja (avtor), Jakše, Jernej (avtor)

Abiotic and biotic stresses can lead to changes in host DNA methylation, which in plants is also mediated by an RNA-directed DNA methylation mechanism. Infections with viroids have been shown to affect DNA methylation dynamics in different plant hosts. The aim of our research was to determine the content of 5-methylcytosine (5-mC) in genomic DNA at the whole genome level of hop plants (Humulus lupulus Var. ‘Celeia’) infected with different viroids and their combinations and to analyse the expression of the selected genes to improve our understanding of DNA methylation dynamics in plant-viroid systems. The adapted HPLC-UV method used proved to be suitable for this purpose, and thus we were able to estimate for the first time that the cytosine methylation level in viroid-free hop plants was 26.7%. Interestingly, the observed 5-mC level was the lowest in hop plants infected simultaneously with CBCVd, HLVd and HSVd (23.7%), whereas the highest level was observed in plants infected with HLVd (31.4%). In addition, we identified three DNA methylases and one DNA demethylase gene in the hop’s draft genome. The RT-qPCR revealed upregulation of all newly identified genes in hop plants infected with all three viroids, while no altered expression was observed in any of the other hop plants tested, except for CBCVd-infected hop plants, in which one DNA methylase was also upregulated.

• Jezik: Angleški jezik
• Ključne besede: DNA methylation, viroid, hop plant, HPLC, DNA methylase, DNA demethylase, phylogeny, RT-qPCR
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Leto izida: 2022
• Št. strani: 16 str.
• Številčenje: no. 16, art. 2592, vol. 11
• PID: 20.500.12556/RUL-139383
• UDK: 577.2
• ISSN pri članku: 2073-4409
• COBISS.SI-ID: 119929347
• Datum objave v RUL: 01.09.2022

Gradivo je del revije

• Naslov: Cells
• Skrajšan naslov: Cells
• Založnik: MDPI
• ISSN: 2073-4409
• COBISS.SI-ID: 519958809

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 19.08.2022

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: metilacija DNA, viroidi, hmelj, DNA demetilaza, filogenija, RNA-usmerjena metilacija DNA

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0077
• Naslov: Kmetijske rastline - genetika in sodobne tehnologije

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: Javni sklad Republike Slovenije za razvoj kadrov in štipendije
• Številka projekta: 200751-53626