The aim of this diploma thesis was the synthesis of peptides using two different methods. Both methods were based on the SPPS (Solid-phase peptide synthesis) principle and used the Fmoc protected amino acids. In the first method, an instrument based on flow SPPS was used for the synthesis, which enables rapid chemical synthesis of peptides. Another method of synthesis was a much slower standard SPPS method using a collection vacuum chamber. We successfully synthesized three peptides containing 17 and 26 amino acids, two of which were identical, but synthesized using a different strategy, once with flow SPPS, the second time manually using a collection vacuum chamber. With flow SPPS, peptides were synthesized in a significantly shorter time, than using a collection vacuum chamber, but these were significantly less clean.
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