Induced pluripotent stem cell (iPSC) line MLi004-A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)

Ropret, Sandra (author), Jeriha, Jakob (author), Sorčan, Tjaša (author), Rogar, Marija (author), Zemljič Jokhadar, Špela (author), McGrath, John A. (author), Ilic, Dusko (author), Liović, Mirjana (author)

We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, plu-ripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi004-A line has been also tested for ability to differentiate into fibroblasts.

• Language: English
• Keywords: stem cell, epidermolysis, collagen VII, recessive dystrophic EB, iPSC line, pluripotency, fibroblast, keratinocyte
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 5 str.
• Numbering: Vol. 55, art. 102463
• PID: 20.500.12556/RUL-138881
• UDC: 61
• ISSN on article: 1876-7753
• DOI: 10.1016/j.scr.2021.102463
• COBISS.SI-ID: 74980611
• Publication date in RUL: 24.08.2022

Record is a part of a journal

• Title: Stem cell research
• Publisher: Elsevier
• ISSN: 1876-7753
• COBISS.SI-ID: 524165401

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: matične celice, epidermoliza, kolagen VII