Microfluidics-based preparation and in situ immobilization of nanoscale cross-linked enzyme aggregates for continuous transamination : research data underlying the article

Menegatti, Tadej (author), Lavrič, Žan (author), Žnidaršič Plazl, Polona (author)

A new microfluidics-based method for the generation and further continuous use of amine transaminase cross-linked enzyme aggregates (ATA-CLEAs) has been developed. The precipitation and cross-linking are carried out in separate but interconnected parts, which allows optimization of each step and thus better final quality of the preparation. After optimizing the acetone and glutaraldehyde concentrations for precipitation and crosslinking, respectively, ATA-CLEAs prepared in a microfluidic device exhibited 87.1% recovered activities, which is 2.4-fold higher than the values previously reported for ATA-CLEAs. The prepared ATA-CLEAs with an average particle radius of 37.13 ± 0.38 nm showed also higher activity than the free enzyme at 70°C. Further integration of a membrane microreactor in the system enabled in situ ATA-CLEA immobilization with 100% yield and up to 68.4% efficiency. The immobilization efficiency and operational stability of the membrane reactor with ATA-CLEAs were more than 20% higher compared to the non-aggregated enzyme.

• Language: English
• Keywords: amine transaminase, enzyme immobilization, cross-linked enzyme aggregates, membrane microreactor, flow biocatalysis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2022
• PID: 20.500.12556/RUL-138145
• Data col. methods: Measurements and tests
• Publication date in RUL: 12.07.2022

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: amin transaminaza, immobilizacija encimov, zamreženi encimski agregati, membranski mikroreaktor, pretočna biokataliza