Detection of herpes simplex and varicella-zoster virus from skin lesions : comparison of RT-PCR and isothermal amplification for rapid identification

Jevšnik Virant, Monika (author), Lusa, Lara (author), Uršič, Tina (author), Glinšek Biškup, Urška (author), Petrovec, Miroslav (author)

We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicella-zoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana® instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa = 1). Concordance between Solana HSV1 + 2/VZV and RT-PCR was 98.3% (kappa = 0.95) for HSV-1 and 99.3% (kappa = 0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.

• Language: English
• Keywords: herpes simplex virus, varicella-zoster virus, skin lesion, isothermal amplification, herpes simplex virus 1, herpes simplex virus 2
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 4 str.
• Numbering: Vol. 97, iss. 2, art. 115015
• PID: 20.500.12556/RUL-137783
• UDC: 616.5
• ISSN on article: 1879-0070
• DOI: 10.1016/j.diagmicrobio.2020.115015
• COBISS.SI-ID: 34704857
• Publication date in RUL: 30.06.2022

Record is a part of a journal

• Title: Diagnostic microbiology and infectious disease
• Publisher: Elsevier
• ISSN: 1879-0070
• COBISS.SI-ID: 23142149

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: herpesvirus, virus varicella zoster, lezija kože, virus herpes simpleks tip 1, virus herpes simpleks tip 2

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0083
• Name: Odnosi parazitskega obstajanja