Antibiotic resistance in bacteria is a growing problem as it grows. This has helped to bring back interest in phage therapy based on the use of bacteriophages to treat bacterial infections in humans. Bacteriophages are bacterial viruses that infect a bacterium and then lyse it at the site of infection. Antibiotic-resistant bacteria include Escerichia coli. Some strains are pathogenic and can cause many health problems and diseases. The objectives of the thesis were to determine the growth parameters of phage T7 in different physiological conditions of the bacterium. They were determined on the basis of a non-invasive method, which included determining parameters in batch conditions of Escherichia coli cultivation, then infecting bacteria with bacteriophages and monitoring their concentration over a period of time. Based on the obtained phage growth curve, we calculated the adsorption constant, determined the length of the latent and eclipse periods, and calculated the multiplication number. Based on the obtained results, it can be argued that the adsorption constant decreases with increasing bacterial growth, the length of the latent and eclipse periods also decreaser, and the burst size is higher.. We can confirm the hypothesis that the physiological state of bacterial culture affects the growth parameters of phage T7.
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