In the first part of the study we collected and analysed antimicrobial resistance data of clinically important anaerobic bacteria in Slovenia isolated in the years 2018, 2019, and 2020. The anaerobic izolates were acquired from the laboratories of the National Laboratory of Health, Environment and Food. We checked the resistance to penicillin, clindamycin, metronidazole, imipenem, and amoxicillin-clavulanic acid. We then collected minimal inhibitory concentration values interpreted according to the latest EUCAST standard edition. We analysed and presented cumulative antimicrobial susceptibility test data in Excel according to the latest CLSI M39 standard edition. The highest resistance rates were for penicillin and clindamycin (40 % and 20% total resistance of tested isolates, respectively). The most resistant isolates were Bacteroides, Prevotella, and Parabacteroides. We found that resistance rates in anaerobes in Slovenia have remained stable in recent years and are comparable to global resistance rates. In the second part of the study, we validated the MICRONAUT-S Anaerobes microdilution method (Merlin Diagnostics, Berlin, Germany) for antimicrobial resistance testing. We tested some of the most clinically important anaerobic bacterial isolates. The aim of this study was to determine whether the MICRONAUT-S Anaerobes microdilution method is suitable for rutine diagnostics in clinical microbiology laboratories. We found that the MICRONAUT-S Anaerobes microdilution method is comparable to the standard gradient-diffusion Etest method and suitable for use in rutine diagnostics.
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