Knee and peri-knee tissues of post mortem donors are strategic sources of mesenchymal stem/stromal cells for regenerative procedures

Haring, Gregor (avtor), Zupan, Janja (avtor)

Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of CD146 in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.

• Jezik: Angleški jezik
• Ključne besede: mesenchymal stem/stromal cells, post mortem donors, knee, peri-knee tissues, bone and bone marrow, synovium, periosteum, in vitro analysis, human skeletal stem cell markers
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 21 str.
• Številčenje: Vol. 23, iss. 6, art. 3170
• PID: 20.500.12556/RUL-137537
• UDK: 602.9:576.7:611.728.3
• ISSN pri članku: 1422-0067
• DOI: 10.3390/ijms23063170
• COBISS.SI-ID: 103163395
• Datum objave v RUL: 21.06.2022

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 15.03.2022

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mezenhimske matične/stromalne celice, darovalci ob smrti, skeletne matične celice, koleno, perikolenska tkiva, kostni mozeg, sinovij, periosteum, in vitro analiza, označevalci človeških skeletnih matičnih celic, matične celice, tkiva

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: J3-1749
• Naslov: Mezenhimske matične celice - nosilci endogene regenerativne sposobnosti tkiv v boju proti staranju mišično-skeletnega sistema