As part of my thesis, we examined the scientific literature in the field of the bacteria Bacillus cereus and their biofilm formation in the dairy industry to explore potential control strategies. The reason for the research was the practice in the microbiological laboratory of Incom d.o.o., where one of the parameters of the analysis was the presence of B. cereus bacteria. These bacteria are Gram-positive, which pose a challenge to the dairy industry due to sporulation, ability of strong adhesion, formation of toxins and extracellular matrix, especially in connection with biofilms. Currently, the most effective removal is the use of the CIP cleaning system with the chemical agents NaOH and HNO3 at 65 °C. In the future, it would be interesting to investigate the efficacy of using antimicrobial coatings and chemicals (e.g., sodium hypochlorite, hydrogen peroxide, ozone, peracetic acid), involving phages, ultrasound, enzymes and a combination of all of these in the hurdle technology. The latter have been used in the literature to control and reduce other bacteria, but present a challenge for the film-forming and sporogenic B. cereus.
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