Evaluation of triploid rainbow trout Oncorhynchus mykiss as a surrogate parent for brown trout Salmo trutta m. fario and grayling Thymallus thymallus

Marinovic, Zoran (avtor), Lujić, Jelena (avtor), Sušnik Bajec, Simona (avtor), Djurdjevič, Ida (avtor), Snoj, Aleš (avtor), Hoitsy, György (avtor), Urbányi, Béla (avtor), Horváth, Ákos G. (avtor)

Surrogate production through germline stem cell (GSC) transplantation is becoming a very promising technology in species management and ex situ species conservation. In the present study, we have evaluated if the triploid rainbow trout is a suitable surrogate parent for the production of other salmonid gametes. In three independent trials conducted in two countries, we have transplanted brown trout and grayling GSCs into triploid rainbow trout larvae as recipients. Two months after transplantation, dissection of recipients displayed that both spermatogonial (SSCs) and oogonial (OSCs) stem cells of both species were able to colonize recipient gonads. After three years of rearing, neither male nor female recipients displayed signs of gametogenesis progression. Species-specific (for both donor and recipient species) amplification of mtDNA control region on the other hand displayed a presence of donor-derived germ cells within recipient gonads. This indicated that even though donor cells were able to colonize recipient gonads, they remained in a dormant or quasi-dormant state, and did not progress with gametogenesis. This study displays that the rainbow trout is not a suitable recipient for all salmonid species, and that careful selection of recipients is a crucial step in developing the surrogate production technology.

• Jezik: Angleški jezik
• Ključne besede: germline stem cells, spermatogonia, oogonia, transplantation, histology
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: 7 str.
• Številčenje: Vol. 24, art. 101163
• PID: 20.500.12556/RUL-137351
• UDK: 579.2/.5
• ISSN pri članku: 2352-5134
• DOI: 10.1016/j.aqrep.2022.101163
• COBISS.SI-ID: 108172803
• Datum objave v RUL: 13.06.2022

Gradivo je del revije

• Naslov: Aquaculture reports
• Založnik: Elsevier
• ISSN: 2352-5134
• COBISS.SI-ID: 525119001

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.
• Začetek licenciranja: 16.05.2022

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: ribe, postrvi, zarodne matične celice, transplantacija

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0220
• Naslov: Primerjalna genomika in genomska biodiverziteta

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: Thematic Excellence Programme 2020, National Challenges Subprogramme
• Številka projekta: TKP2020-NKA-16