Biostimulatory effects of amino acids on phenylalanine ammonia lyase, capsaicin synthase, and peroxidase activities in Capsicum baccatum L.

Zamljen, Tilen (author), Medič, Aljaž (author), Hudina, Metka (author), Veberič, Robert (author), Slatnar, Ana (author)

Biostimulants are widely used in agriculture because they can improve fruit quality and quantity. Less is known about how biostimulants act over time in plants, in our case peppers, and how they affect the enzyme activity of important enzymes for capsaicinoid synthesis. The biostimulatory effects of amino acids on the activities of phenylalanine ammonia lyase (PAL), capsaicin synthase (CS), and peroxidase (POX) were investigated in the pericarp and placenta of the chili pepper Capsicum baccatum L. cv. “Bishop Crown” over 72 h of application. The PAL and CS activities significantly increased in the placenta after 1 h of biostimulant application, with significant increases of 130% and 16%, respectively. The POX activity remained unchanged over the full 72 h in the placenta but significantly increased after 48 h in the pericarp (+53%). Total capsaicinoids increased in the first hour of biostimulant application, by 4.30 g/kg FW in the placenta (19%) and by 0.94 g/kg FW in the pericarp (+56%). Biostimulant application also increased total and individual capsaicinoids after 48 h in the chili placenta and pericarp. With improved methods for enzymatic determination, we gained new insights into the responses of chilies to biostimulant amino acids.

• Language: English
• Keywords: biostimulants, capsaicin, enzyme, trans-cinnamic acid, time course, amino acids, Capsicum baccatum
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 11 str.
• Numbering: Vol. 11, iss. 5, art. 674
• PID: 20.500.12556/RUL-136450
• UDC: 634.5
• ISSN on article: 2079-7737
• DOI: 10.3390/biology11050674
• COBISS.SI-ID: 106540035
• Publication date in RUL: 04.05.2022

Record is a part of a journal

• Title: Biology
• Shortened title: Biology
• Publisher: MDPI
• ISSN: 2079-7737
• COBISS.SI-ID: 523004441

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: biostimulanti, Capsicum baccatum, kapsaicin, encimi, trans-cimetova kislina

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0013
• Name: Hortikultura

• Funder: ARRS - Slovenian Research Agency
• Funding programme: Infrastructural center
• Project number: IO-0022-0481-001
• Acronym: IC RRC-AG