Solubility of luteolin and other polyphenolic compounds in water, nonpolar, polar aprotic and protic solvents by applying FTIR/HPLC

Rajhard, Saša (author), Hladnik, Lucija (author), Vicente, Filipa A. (author), Srčič, Stanko (author), Grilc, Miha (author), Likozar, Blaž (author)

In recent years, flavonoids have become a highly researched topic due to their health beneficial effects. Since flavonoids’ solubility plays a significant role in their use in pharmaceutical, food, biological, and chemical areas, the determination of suitable solvents is crucial. Fourier transform infrared (FTIR) analysis was used to characterize functional groups of several flavonoids and phenolic compounds, namely luteolin, hesperidin, quercetin, naringenin, gallic acid and tannic acid. Concentration dependence on transmittance was evaluated for these compounds in ethanol. Afterwards, luteolin was chosen as a model flavonoid, with its concentration correlated with transmittance using 15 solvents with different polarities. Luteolin solubility was further corroborated with high-performance liquid chromatography (HPLC). These results shed light on using FTIR as a semi-quantitative method for the initial screening of solvents and the solubility of different compounds while saving time and solvents. Hence, HPLC would only be needed as a final step for the most promising solvents.

• Language: English
• Keywords: luteolin, dissolution, fourier transform infrared-attenuated total reflectance (FTIR-ATR) spectroscopy, fast quantification tool, high-performance liquid chromatography (HPLC)
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 15 str.
• Numbering: Vol. 9, iss. 11, art. 1952
• PID: 20.500.12556/RUL-136428
• UDC: 577
• ISSN on article: 2227-9717
• DOI: 10.3390/pr9111952
• COBISS.SI-ID: 85080835
• Publication date in RUL: 03.05.2022

Record is a part of a journal

• Title: Processes
• Shortened title: Processes
• Publisher: MDPI AG
• ISSN: 2227-9717
• COBISS.SI-ID: 523353113

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.11.2021

Secondary language

• Language: Slovenian
• Keywords: biokemija, FTIR, flavonoidi, Fourierjeva transformacija