Isolation of chromatophores from brown trout (Salmo trutta) skin

Renko, Gašper (author), Djurdjevič, Ida (author), Sušnik Bajec, Simona (author)

As new techniques for single-cell analysis are evolving, there is a growing need for streamlined and flexible protocols for single-cell suspension preparation and single-cell isolation. Based on previous research on the model species zebrafish (Danio rerio), a novel protocol for the isolation of chromatophores from brown trout (Salmo trutta) skin was developed and specific gene expression in chromatophore types evaluated with qPCR. This is the first report on a protocol for the isolation of chromatophores from the skin of salmonids. The method is simple and fast (taking about 90 min), requires no special equipment and is in line with the high ethical standards required in animal research, as no animals need to be sacrificed. In addition, skin biopsies can be taken from the same fish at multiple time points, allowing monitoring of chromatophore differentiation and their involvement in the formation of pigmentation patterns. The protocol should be effective for the vast majority of salmonid species due to similarities in skin morphology.

• Language: English
• Keywords: melanophore, erythrophore, single cell, qPCR
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2022
• Number of pages: 13 str.
• Numbering: Vol. 7, iss. 2, art. 72
• PID: 20.500.12556/RUL-136357
• UDC: 579.2/.5
• ISSN on article: 2410-3888
• DOI: 10.3390/fishes7020072
• COBISS.SI-ID: 101735683
• Publication date in RUL: 25.04.2022

Record is a part of a journal

• Title: Fishes
• Shortened title: Fishes
• Publisher: MDPI
• ISSN: 2410-3888
• COBISS.SI-ID: 526775577

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 01.04.2022

Secondary language

• Language: Slovenian
• Keywords: ribe, postrvi, potočna postrv, koža, pigmentacija, kromatofore, izolacija, metode, qPCR

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0220
• Name: Primerjalna genomika in genomska biodiverziteta