Identification and quantification of naphthoquinones and other phenolic compounds in leaves, petioles, bark, roots, and buds of Juglans regia L., using HPLC-MS/MS

Medič, Aljaž (author), Zamljen, Tilen (author), Hudina, Metka (author), Veberič, Robert (author)

The present study was designed to identify and quantify the major phenolic compounds in different Juglans regia L. (common walnut) tissues (leaves, petioles, bark, roots, buds), to define the compositions and contents of phenolic compounds between these tissues. A total of 91 individual phenolic compounds were identified and quantified, which comprised 8 hydroxycinnamic acids, 28 hydroxybenzoic acids, 11 flavanols, 20 flavonols, 22 napthoquinones, and 2 coumarins. Naphthoquinones were the major phenolic group in leaves, petioles, bark, and buds, as >60% of those identified, while hydroxybenzoic acids were the major phenolic group in side roots, as ~50% of those identified. The highest content of phenolic compounds was in the J. regia main root, followed by side roots and buds, leaves, and 1-year-old bark; the lowest content was in petioles and 2-year-old bark. Leaves, roots, and buds of J. regia represent a valuable source of these agro-residues.

• Language: English
• Keywords: Juglans regia L., walnut, leaf, petiole, bark, root, bud, phenolic compounds, naphthoquinones
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 13 str.
• Numbering: Vol. 7, iss. 9, art. 326
• PID: 20.500.12556/RUL-136341
• UDC: 634.1/.7
• ISSN on article: 2311-7524
• DOI: 10.3390/horticulturae7090326
• COBISS.SI-ID: 77587203
• Publication date in RUL: 25.04.2022

MEDIČ, Aljaž, ZAMLJEN, Tilen, HUDINA, Metka and VEBERIČ, Robert, 2021, Identification and quantification of naphthoquinones and other phenolic compounds in leaves, petioles, bark, roots, and buds of Juglans regia L., using HPLC-MS/MS. Horticulturae [online]. 2021. Vol. 7, no. 9,  326. [Accessed 14 April 2025]. DOI 10.3390/horticulturae7090326. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=136341

Record is a part of a journal

• Title: Horticulturae
• Publisher: MDPI
• ISSN: 2311-7524
• COBISS.SI-ID: 526326297

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 18.09.2021

Secondary language

• Language: Slovenian
• Keywords: oreh, Juglans regia, kemična sestava, sekundarni metaboliti, fenolne spojine

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0013
• Name: Hortikultura