Current technologies for RNA-directed liquid diagnostics

Martinez-Dominguez, Maria Victoria (author), Zottel, Alja (author), Šamec, Neja (author), Jovchevska, Ivana (author), Dincer, Can (author), Kahlert, Ulf Dietrich (author), Nickel, Ann-Christin (author)

There is unequivocal acceptance of the variety of enormous potential liquid nucleic acid-based diagnostics seems to offer. However, the existing controversies and the increased awareness of RNA-based techniques in society during the current global COVID-19 pandemic have made the readiness of liquid nucleic acid-based diagnostics for routine use a matter of concern. In this regard—and in the context of oncology—our review presented and discussed the status quo of RNA-based liquid diagnostics. We summarized the technical background of the available assays and benchmarked their applicability against each other. Herein, we compared the technology readiness level in the clinical context, economic aspects, implementation as part of routine point-of-care testing as well as performance power. Since the preventive care market is the most promising application sector, we also investigated whether the developments predominantly occur in the context of early disease detection or surveillance of therapy success. In addition, we provided a careful view on the current biotechnology investment activities in this sector to indicate the most attractive strategies for future economic success. Taken together, our review shall serve as a current reference, at the interplay of technology, clinical use and economic potential, to guide the interested readers in this rapid developing sector of precision medicine.

• Language: English
• Keywords: liquid biopsy, circulating free nucleic acids, exosome, cancer biomarker
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 26 str.
• Numbering: Vol. 13, iss. 20, art. 5060
• PID: 20.500.12556/RUL-136264
• UDC: 616-006
• ISSN on article: 2072-6694
• DOI: 10.3390/cancers13205060
• COBISS.SI-ID: 80452099
• Publication date in RUL: 21.04.2022

Record is a part of a journal

• Title: Cancers
• Shortened title: Cancers
• Publisher: MDPI
• ISSN: 2072-6694
• COBISS.SI-ID: 517914137

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 10.10.2021

Secondary language

• Language: Slovenian
• Keywords: tekoča biopsija, proste nukleinske kisline v obtoku, eksosom