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Perspectives of microscopy methods for morphology characterisation of extracellular vesicles from human biofluids
ID
Malenica, Mladenka
(
Avtor
),
ID
Wechtersbach, Karmen
(
Avtor
),
ID
Kojc, Nika
(
Avtor
), et al.
PDF - Predstavitvena datoteka,
prenos
(1,81 MB)
MD5: A78E92DB0C9FE3F110E122233D0EE6AA
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/2227-9059/9/6/603
Galerija slik
Izvleček
Extracellular vesicles (EVs) are nanometric membranous structures secreted from almost every cell and present in biofluids. Because EV composition reflects the state of its parental tissue, EVs possess an enormous diagnostic/prognostic potential to reveal pathophysiological conditions. However, a prerequisite for such usage of EVs is their detailed characterisation, including visualisation which is mainly achieved by atomic force microscopy (AFM) and electron microscopy (EM). Here we summarise the EV preparation protocols for AFM and EM bringing out the main challenges in the imaging of EVs, both in their natural environment as biofluid constituents and in a saline solution after EV isolation. In addition, we discuss approaches for EV imaging and identify the potential benefits and disadvantages when different AFM and EM methods are applied, including numerous factors that influence the morphological characterisation, standardisation, or formation of artefacts. We also demonstrate the effects of some of these factors by using cerebrospinal fluid as an example of human biofluid with a simpler composition. Here presented comparison of approaches to EV imaging should help to estimate the current state in morphology research of EVs from human biofluids and to identify the most efficient pathways towards the standardisation of sample preparation and microscopy modes.
Jezik:
Angleški jezik
Ključne besede:
extracellular vesicles
,
human biofluids
,
nanotechnology
,
atomic force microscopy
,
electron microscopy
,
morphology
Vrsta gradiva:
Članek v reviji
Tipologija:
1.02 - Pregledni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2021
Št. strani:
29 str.
Številčenje:
Vol. 9, iss. 6, art. 603
PID:
20.500.12556/RUL-135645
UDK:
54
ISSN pri članku:
2227-9059
DOI:
10.3390/biomedicines9060603
COBISS.SI-ID:
64802819
Datum objave v RUL:
23.03.2022
Število ogledov:
877
Število prenosov:
89
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Biomedicines
Skrajšan naslov:
Biomedicines
Založnik:
MDPI AG
ISSN:
2227-9059
COBISS.SI-ID:
523006745
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
01.06.2021
Projekti
Financer:
EC - European Commission
Program financ.:
H2020
Številka projekta:
654360
Naslov:
Nanoscience foundries and fine analysis - Europe
Akronim:
NFFA-Europe
Financer:
HRZZ - Croatian Science Foundation
Številka projekta:
IP-2019-04-1511
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
University of Rijeka
Številka projekta:
uniri-biomed-18-279
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
University of Rijeka
Številka projekta:
uniri-biomed-18-5
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
University of Rijeka
Številka projekta:
uniri-tehnik-18-294
Financer:
EC - European Commission
Program financ.:
European Regional Development Fund
Naslov:
Research Infrastructure for Campus-based Laboratories at the University of Rijeka
Financer:
MZOS - Ministry of Science, Education and Sports of the Republic of Croatia
Številka projekta:
RC.2.2.06-0001
Naslov:
Research Infrastructure for Campus-based Laboratories at the University of Rijeka
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P2-0091
Naslov:
Sodobni anorganski materiali in nanotehnologije
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
N2-0150
Naslov:
Elektrostimulatorji z vizualno detekcijo mehanske deformacije in napetosti: inovativni piezoelektrični biomateriali za elektro-stimulirano celično rast
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