Pulsed electric field (PEF) enhances iron uptake by the yeast Saccharomyces cerevisiae

Nowosad, Karolina (author), Sujka, Monika (author), Pankiewicz, Urszula (author), Miklavčič, Damijan (author), Arczewska, Marta (author)

The aim of the study was to investigate the influence of a pulsed electric field (PEF) on the level of iron ion accumulation in Saccharomyces cerevisiae cells and to select PEF conditions optimal for the highest uptake of this element. Iron ions were accumulated most efficiently when their source was iron (III) nitrate. When the following conditions of PEF treatment were used: voltage 1500 V, pulse width 10 µs, treatment time 20 min, and a number of pulses 1200, accumulation of iron ions in the cells from a 20 h-culture reached a maximum value of 48.01 mg/g dry mass. Application of the optimal PEF conditions thus increased iron accumulation in cells by 157% as compared to the sample enriched with iron without PEF. The second derivative of the FTIR spectra of iron-loaded and -unloaded yeast cells allowed us to determine the functional groups which may be involved in metal ion binding. The exposure of cells to PEF treatment only slightly influenced the biomass and cell viability. However, iron-enriched yeast (both with or without PEF) showed lower fermentative activity than a control sample. Thus obtained yeast biomass containing a high amount of incorporated iron may serve as an alternative to pharmacological supplementation in the state of iron deficiency.

• Language: English
• Keywords: iron accumulation, yeast, iron deficiency, PEF
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 19 str.
• Numbering: Vol. 11, iss. 6, art. 850
• PID: 20.500.12556/RUL-135644
• UDC: 577:621.3
• ISSN on article: 2218-273X
• DOI: 10.3390/biom11060850
• COBISS.SI-ID: 66394115
• Publication date in RUL: 23.03.2022

Record is a part of a journal

• Title: Biomolecules
• Shortened title: Biomolecules
• Publisher: MDPI
• ISSN: 2218-273X
• COBISS.SI-ID: 519952921

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 07.06.2021

Secondary language

• Language: Slovenian
• Keywords: kopičenje železa, kvas, pomanjkanje železa, PEF