Novel Siphoviridae bacteriophages infecting Bacteroides uniformis contain diversity generating retroelement

Hedžet, Stina (avtor), Rupnik, Maja (avtor), Accetto, Tomaž (avtor)

Intestinal phages are abundant and important components of gut microbiota, yet the isolated and characterized representatives that infect abundant gut bacteria are sparse. Here we describe the isolation of human intestinal phages infecting Bacteroides uniformis. Bacteroides is one of the most common bacterial groups in the global human gut microbiota; however, to date not many Bacteroides specific phages are known. Phages isolated in this study belong to a novel viral genus, Bacuni, within the Siphoviridae family. Their genomes encode diversity-generating retroelements (DGR), which were shown in other bacteriophages to promote phage adaptation to rapidly changing environmental conditions and to broaden their host range. Three isolated phages showed 99.83% genome identity but one of them infected a distinct B. uniformis strain. The tropism of Bacuni phages appeared to be dependent on the interplay of DGR mediated sequence variations of gene encoding putative phage fimbrial tip proteins and mutations in host genes coding for outer-membrane proteins. We found prophages with up to 85% amino acid similarity over two-thirds of the Bacuni phage genome in the B. acidifaciens and Prevotella sp. genomes. Despite the abundance of Bacteroides within the human microbiome, we found Bacuni phages only in a limited subset of published gut metagenomes.

• Jezik: Angleški jezik
• Ključne besede: gut, Bacteroides, virome, prophage, diversity-generating retroelement
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 16 str.
• Številčenje: Vol. 9, iss. 5, art. 892
• PID: 20.500.12556/RUL-135541
• UDK: 579
• ISSN pri članku: 2076-2607
• DOI: 10.3390/microorganisms9050892
• COBISS.SI-ID: 61197059
• Datum objave v RUL: 18.03.2022

Gradivo je del revije

• Naslov: Microorganisms
• Skrajšan naslov: Microorganisms
• Založnik: MDPI AG
• ISSN: 2076-2607
• COBISS.SI-ID: 523277081

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 01.05.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikrobiologija, bakterije, Bacteroides uniformis, Siphoviridae, bakteriofagi, črevesje, mikrobiota