Kin discrimination drives territorial exclusion during Bacillus subtilis swarming and restrains exploitation of surfactin

Kraigher, Barbara (avtor), Butolen, Monika (avtor), Štefanič, Polonca (avtor), Mandić-Mulec, Ines (avtor)

Swarming is the collective movement of bacteria across a surface. It requires the production of surfactants (public goods) to overcome surface tension and provides an excellent model to investigate bacterial cooperation. Previously, we correlated swarm interaction phenotypes with kin discrimination between B. subtilis soil isolates, by showing that less related strains form boundaries between swarms and highly related strains merge. However, how kin discrimination affects cooperation and territoriality in swarming bacteria remains little explored. Here we show that the pattern of surface colonization by swarming mixtures is influenced by kin types. Closely related strain mixtures colonize the surface in a mixed swarm, while mixtures of less related strains show competitive exclusion as only one strain colonizes the surface. The outcome of nonkin swarm expansion depends on the initial ratio of the competing strains, indicating positive frequency-dependent competition. We find that addition of surfactin (a public good excreted from cells) can complement the swarming defect of nonkin mutants, whereas close encounters in nonkin mixtures lead to territorial exclusion, which limits the exploitation of surfactin by nonkin nonproducers. The work suggests that kin discrimination driven competitive territorial exclusion may be an important determinant for the success of cooperative surface colonization.

• Jezik: Angleški jezik
• Ključne besede: Bacillus subtilis, swaraming, social interactions, surfactin, biofilms, microbial ecology
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2022
• Št. strani: Str. 833–841
• Številčenje: Vol. 16, iss. 3
• PID: 20.500.12556/RUL-135330
• UDK: 579.22/.26:579.852.11
• ISSN pri članku: 1751-7362
• DOI: 10.1038/s41396-021-01124-4
• COBISS.SI-ID: 80968963
• Datum objave v RUL: 08.03.2022

Gradivo je del revije

• Naslov: The ISME journal
• Skrajšan naslov: ISME j.
• Založnik: Springer Nature, International Society for Microbial Ecology
• ISSN: 1751-7362
• COBISS.SI-ID: 513948953

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Bacillus subtilis, rojenje, socialne interakcije, surfaktin