Stressor-dependant changes in immune parameters in the terrestrial isopod crustacean, Porcellio scaber : a focus on nanomaterials

Mayall, Craig (avtor), Dolar, Andraž (avtor), Jemec Kokalj, Anita (avtor), Novak, Sara (avtor), Razinger, Jaka (avtor), Barbero, Francesco (avtor), Puntes, Victor (avtor), Drobne, Damjana (avtor)

We compared the changes of selected immune parameters of Porcellio scaber to different stressors. The animals were either fed for two weeks with Au nanoparticles (NPs), CeO$_2$ NPs, or Au ions or body-injected with Au NPs, CeO$_2$ NPs, or lipopolysaccharide endotoxin. Contrary to expectations, the feeding experiment showed that both NPs caused a significant increase in the total haemocyte count (THC). In contrast, the ion-positive control resulted in a significantly decreased THC. Additionally, changes in phenoloxidase (PO)-like activity, haemocyte viability, and nitric oxide (NO) levels seemed to depend on the stressor. Injection experiments also showed stressor-dependant changes in measured parameters, such as CeO$_2$ NPs and lipopolysaccharide endotoxin (LPS), caused more significant responses than Au NPs. These results show that feeding and injection of NPs caused an immune response and that the response differed significantly, depending on the exposure route. We did not expect the response to ingested NPs, due to the low exposure concentrations (100 µg/g dry weight food) and a firm gut epithelia, along with a lack of phagocytosis in the digestive system, which would theoretically prevent NPs from crossing the biological barrier. It remains a challenge for future research to reveal what the physiological and ecological significance is for the organism to sense and respond, via the immune system, to ingested foreign material.

• Jezik: Angleški jezik
• Ključne besede: gold nanoparticles, cerium nanoparticles, woodlice, immune response, haemocyte
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2021
• Št. strani: 18 str.
• Številčenje: Vol. 11, iss. 4, art. 934
• PID: 20.500.12556/RUL-135230
• UDK: 577
• ISSN pri članku: 2079-4991
• DOI: 10.3390/nano11040934
• COBISS.SI-ID: 59056899
• Datum objave v RUL: 02.03.2022

Gradivo je del revije

• Naslov: Nanomaterials
• Skrajšan naslov: Nanomaterials
• Založnik: MDPI AG
• ISSN: 2079-4991
• COBISS.SI-ID: 523286297

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
• Začetek licenciranja: 06.04.2021

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: imunski odziv, kopenski raki, nanodelci, spremembe imunskih parametrov

Projekti

• Financer: EC - European Commission
• Program financ.: H2020
• Številka projekta: 671881
• Naslov: Probing safety of nano-objects by defining immune responses of environmental organisms
• Akronim: PANDORA