Core RNA interference genes involved in miRNA and ta-siRNA biogenesis in hops and their expression analysis after challenging with Verticillium nonalfalfae

Kunej, Urban (author), Jakše, Jernej (author), Radišek, Sebastjan (author), Štajner, Nataša (author)

RNA interference is an evolutionary conserved mechanism by which organisms regulate the expression of genes in a sequence-specific manner to modulate defense responses against various abiotic or biotic stresses. Hops are grown for their use in brewing and, in recent years, for the pharmaceutical industry. Hop production is threatened by many phytopathogens, of which Verticillium, the causal agent of Verticillium wilt, is a major contributor to yield losses. In the present study, we performed identification, characterization, phylogenetic, and expression analyses of three Argonaute, two Dicer-like, and two RNA-dependent RNA polymerase genes in the susceptible hop cultivar Celeia and the resistant cultivar Wye Target after infection with Verticillium nonalfalfae. Phylogeny results showed clustering of hop RNAi proteins with their orthologues from the closely related species Cannabis sativa, Morus notabilis and Ziziphus jujuba which form a common cluster with species of the Rosaceae family. Expression analysis revealed downregulation of argonaute 2 in both cultivars on the third day post-inoculation, which may result in reduced AGO2-siRNA-mediated posttranscriptional gene silencing. Both cultivars may also repress ta-siRNA biogenesis at different dpi, as we observed downregulation of argonaute 7 in the susceptible cultivar on day 1 and downregulation of RDR6 in the resistant cultivar on day 3 after inoculation.

• Language: English
• Keywords: Humulus lupulus, Verticillium nonalfalfae, fungal infection, RNA interference, plant–pathogen interactions, small RNA
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 16 str.
• Numbering: Vol. 22, iss. 8, art. 4224
• PID: 20.500.12556/RUL-135182
• UDC: 577.2
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms22084224
• COBISS.SI-ID: 60443139
• Publication date in RUL: 28.02.2022

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 19.04.2021

Secondary language

• Language: Slovenian
• Keywords: Humulus lupulus, Verticillium nonalfalfae, verticilijska uvelost, glivična okužba, interferenca RNA, interakcija rastlina-patogen

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: 34110

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0077
• Name: Kmetijske rastline - genetika in sodobne tehnologije