Adipose tissue gene expression of entire male, immunocastrated and surgically castrated pigs

Poklukar Žnidaršič, Klavdija (author), Čandek-Potokar, Marjeta (author), Vrecl, Milka (author), Batorek Lukač, Nina (author), Fazarinc, Gregor (author), Kress, Kevin (author), Stefanski, Volker (author), Škrlep, Martin (author)

Differences in adipose tissue deposition and properties between pig male sex categories, i.e., entire males (EM), immunocastrates (IC) and surgical castrates (SC) are relatively well-characterized, whereas the underlying molecular mechanisms are still not fully understood. To gain knowledge about the genetic regulation of the differences in adipose tissue deposition, two different approaches were used: RNA-sequencing and candidate gene expression by quantitative PCR. A total of 83 differentially expressed genes were identified between EM and IC, 15 between IC and SC and 48 between EM and SC by RNA-sequencing of the subcutaneous adipose tissue. Comparing EM with IC or SC, upregulated genes related to extracellular matrix dynamics and adipogenesis, and downregulated genes involved in the control of lipid and carbohydrate metabolism were detected. Differential gene expression generally indicated high similarity between IC and SC as opposed to EM, except for several heat shock protein genes that were upregulated in EM and IC compared with SC. The candidate gene expression approach showed that genes involved in lipogenesis were downregulated in EM compared with IC pigs, further confirming RNA-sequencing results.

• Language: English
• Keywords: pigs, adipose tissue, entire males, immunocastration, surgical castration, RNA-sequencing, expression
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 18 str.
• Numbering: Vol. 22, iss. 4, art. 1768
• PID: 20.500.12556/RUL-135038
• UDC: 636.4
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms22041768
• COBISS.SI-ID: 50997507
• Publication date in RUL: 18.02.2022

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 10.02.2021

Secondary language

• Language: Slovenian
• Keywords: prašiči, maščobno tkivo, samci, imunokastracija, kirurška kastracija