Effect of hydrolyzable tannins on glucose-transporter expression and their bioavailability in pig small-intestinal 3D cell model

Brus, Maksimiljan (author), Frangež, Robert (author), Gorenjak, Mario (author), Kotnik, Petra (author), Knez, Željko (author), Škorjanc, Dejan (author)

Intestinal transepithelial transport of glucose is mediated by glucose transporters, and affects postprandial blood-glucose levels. This study investigates the effect of wood extracts rich in hydrolyzable tannins (HTs) that originated from sweet chestnut (Castanea sativa Mill.) and oak (Quercus petraea) on the expression of glucose transporter genes and the uptake of glucose and HT constituents in a 3D porcine-small-intestine epithelial-cell model. The viability of epithelial cells CLAB and PSI exposed to different HTs was determined using alamarBlue$^®$. qPCR was used to analyze the gene expression of SGLT1, GLUT2, GLUT4, and POLR2A. Glucose uptake was confirmed by assay, and LC–MS/ MS was used for the analysis of HT bioavailability. HTs at 37 µg/mL were found to adversely affect cell viability and downregulate POLR2A expression. HT from wood extract Tanex at concentrations of 4 µg/mL upregulated the expression of GLUT2, as well as glucose uptake at 1 µg/mL. The time-dependent passage of gallic acid through enterocytes was influenced by all wood extracts compared to gallic acid itself as a control. These results suggest that HTs could modulate glucose uptake and gallic acid passage in the 3D cell model.

• Language: English
• Keywords: hydrolyzable tannin, glucose, glucose transporters, porcine epithelial cell line, tannin bioavailability, glucose transport
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2021
• Number of pages: 17 str.
• Numbering: Vol. 26, iss. 2, art. 345
• PID: 20.500.12556/RUL-134855
• UDC: 577
• ISSN on article: 1420-3049
• DOI: 10.3390/molecules26020345
• COBISS.SI-ID: 46450435
• Publication date in RUL: 04.02.2022

Record is a part of a journal

• Title: Molecules
• Shortened title: Molecules
• Publisher: MDPI
• ISSN: 1420-3049
• COBISS.SI-ID: 18462981

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 11.01.2021

Secondary language

• Language: Slovenian
• Keywords: glukoza, tanini, biokemija