Determining the molecular background of endometrial receptivity in adenomyosis

Prašnikar, Erika (author), Kunej, Tanja (author), Repnik, Katja (author), Potočnik, Uroš (author), Knez, Jure (author), Kovačič, Borut (author)

Background: Adenomyosis is a gynaecological condition with limited evidence of negative impact to endometrial receptivity. It is commonly associated with endometriosis, which has been shown to alter endometrial expression patterns. Therefore, the candidate genes identified in endometriosis could serve as a source to study endometrial function in adenomyosis. Methods: Transcripts/proteins associated with endometrial receptivity in women with adenomyosis or endometriosis and healthy women were obtained from publications and their nomenclature was adopted according to the HUGO Gene Nomenclature Committee (HGNC). Retrieved genes were analysed for enriched pathways using Cytoscape/Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and Reactome tools to prioritise candidates for endometrial receptivity. These were used for validation on women with (n = 9) and without (n = 13) adenomyosis. Results: Functional enrichment analysis of 173, 42 and 151 genes associated with endometriosis, adenomyosis and healthy women, respectively, revealed signalling by interleukins and interleukin-4 and interleukin-13 signalling pathways, from which annotated LIF, JUNB, IL6, FOS, IL10 and SOCS3 were prioritised. Selected genes showed downregulated expression levels in adenomyosis compared to the control group, but without statistical significance. Conclusion: This is the first integrative study providing putative candidate genes and pathways characterising endometrial receptivity in women with adenomyosis in comparison to healthy women and women with endometriosis.

• Language: English
• Keywords: candidate genes, endometrial receptivity, gene set enrichment analysis (GSEA), multi-omics, protein-protein interaction network (PPIN), adenomyosis, endometriosis, gene expression, genetic association studies
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2020
• Number of pages: 25 str.
• Numbering: Vol. 10, iss. 9, art. 1311
• PID: 20.500.12556/RUL-134656
• UDC: 618.14-002
• ISSN on article: 2218-273X
• DOI: 10.3390/biom10091311
• COBISS.SI-ID: 28211459
• Publication date in RUL: 24.01.2022

Record is a part of a journal

• Title: Biomolecules
• Shortened title: Biomolecules
• Publisher: MDPI
• ISSN: 2218-273X
• COBISS.SI-ID: 519952921

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 11.09.2020

Secondary language

• Language: Slovenian
• Keywords: endometrioza, genetske asociacijske študije, gensko izražanje, adenomioza

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: P3-0327
• Name: Reprodukcija človeka - laboratorijski in eksperimentalni vidiki

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0220
• Name: Primerjalna genomika in genomska biodiverziteta